猪胎儿卵母细胞发生早期的细胞凋亡变化  

Apoptosis Change of the Cells on Fetal Porcine Ovaries

作　　者：霍道坦[1] 陶勇[1] 王勇 权青[1] 田宁宁[1] 成志军[1] 章孝荣[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽安泰农业开发有限责任公司,宣城242200

出　　处：《华中农业大学学报》2010年第1期71-74,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30600432)资助

摘　　要：为研究猪卵母细胞发生早期卵巢上细胞的凋亡情况,制作妊娠33、40、46、54、61 d母猪胎儿卵巢的石蜡切片,并进行荧光染色。结果发现,母猪妊娠到33 d时,胎猪卵巢上即可见原始生殖细胞和卵原细胞的凋亡,随着妊娠时间的延长,凋亡也逐渐增加,妊娠33、40、46、54和61 d母猪胎儿卵巢上卵原细胞的凋亡率分别为3.13%、4.32%、5.50%、9.43%和11.10%,同时,颗粒细胞的凋亡率则分别为8.89%、12.45%、14.74%、17.96%和22.81%。结果说明,在猪胎儿卵母细胞发生早期,即开始发生了卵原细胞和颗粒细胞的凋亡,且有逐渐增加的趋势。Oogenesis is an important physiological event in the process of reproductive development. The present study was undertaken to investigate the apoptosis of the cells on fetal porcine ovaries. The fetal porcine ovaries 33,40,46,54 and 61 days post coitum （dpc） were collected from pregnant sows, respectively,and the paraffin sections were prepared and then stained by fluorescent dye Hoechst 33258. The results showed that the apoptotic cells were observed since 33 dpe in primordial germ cells and oogo- nium,and the apoptosis rates were 3. 13%,4. 32%,5.50%,9.43% and 11.10% at 33,40,46,54 and 61 dpc respectively. Meanwhile, the apoptosis rates of granulosa cells were 8. 89%,12. 45%, 14. 74%, 17.96% and 22.81% at 33,40,46,54 and 61 dpc respectively. These results suggest that the apoptosis of oogonium and follicle cells on fetal porince ovaries happens and increases at the early stage of oogenesis.

关 键 词：胎儿 猪 卵巢 卵原细胞 凋亡 

分 类 号：Q954.4[生物学—动物学]