萝卜-芥蓝异源四倍体F_4和F_(10)世代DNA甲基化变异的MSAP分析  

Methylation Variation of Different Generations of Artificial Raphanus sativus×Brassica alboglabra Allopolyploids Analysed with MSAP

作　　者：李象松[1] 魏丽华[1] 李炫丽[1] 汪艳杰[1] 王冰[1] 吴江生[2] 龙鸿[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2010年第1期96-100,共5页Journal of Huazhong Agricultural University

基　　金：教育部留学回国人员基金项目(4002-082017)资助

摘　　要：以人工合成的萝卜-芥蓝异源四倍体的F4和F10世代为材料,用MSAP法检测2种材料DNA甲基化变异情况,以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据。结果表明:F4代和F10代甲基化程度分别为30.61%和33.10%,共检测12种甲基化变异模式,可以分为5种类型,甲基化变异位点占所有甲基化位点的41.71%。用甲基化抑制剂5-氮胞苷处理F4代,F10代材料,检测到甲基化程度分别为20.67%和25.75%,F代比F代受到了更大冲击,说明不稳定世代对环境的影响更加敏感。Methylation variations of artificial F4 andF10 generations of Raphanus sativus × Brassica alboglabra allopolyploid were examined with MSAP. The results showed that the methylation levels of F4 ,F10were 30. 61% and 33. 10%, respectively. Twelve different models of methylation variations were detected,and these models could be divided into 5 types, with 41.71% variable methylation sites. The methylation levels of F4 and F10 after 5-azacytidine treatment were 20. 67% and 25.75%, respectively. The results showed that after-5-azacytidine treatment, methylation variations of F10 were stronger than those of F4.

关 键 词：MSAP 异源多倍体 DNA甲基化 5-氮胞苷 

分 类 号：S631.1[农业科学—蔬菜学] S565.4[农业科学—园艺学]