ADMA对人脐静脉内皮细胞粘附功能影响的体外研究  

Effects of asymmetrical dimethylarginine on adhension function of human umbilical vein endothelial cells in vitro

作　　者：赵雷[1] 张吉凤 赵佳怡[3] 祝金明 李淑梅[1] 

机构地区：[1]吉林大学第二医院心血管内科,吉林长春130041 [2]吉林再生医学科学研究所 [3]吉化总医院心血管内科

出　　处：《中国实验诊断学》2010年第2期185-187,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察非对称性二甲基精氨酸(ADMA)对体外培养的人脐静脉内皮细胞(HUVECs)粘附功能的影响。方法原代获取HUVECs,分别加入ADMA5μmol/L和10μmol/L培养48小时。测定细胞的数目、粘附和产生一氧化氮的能力。流式细胞仪检测细胞表面粘附分子的表达。结果与对照组相比,不同浓度的ADMA可显著抑制HU-VECs产生NO和粘附的能力、增强细胞表面ICAM-1和VCAM-1的表达。结论ADMA可抑制体外培养的HUVECs粘附功能。Objective To investigate effects of asymmetrical dimethylarginie（ADMA） on adhension function of human umbilical vein endothelial cells（HUVECs）.Methods HUVECs were cultured with ADMA（5 μmol,10 μmol/L）for 48 h in vitro. The adherent cells were counted by microscope. NO production was assayed by Griess nitric oxide assay kit. Intercellular adhension molecule- 1 （ICAM- 1 ） and vascular cell adhension molecule- 1 （VCAM- 1 ） were identified by flow cytometer. Results Compared with control group, ADMA can reduce the number of HUVECs and inhibit the function of excreting NO. ADMA can enhance the expression of ICAM- 1 and VCAM-1 on the surface of HUVECs. Conclusion ADMA can inhibit adhension function of HUVECs in vitro.

关 键 词：非对称性二甲基精氨酸 冠状动脉粥样硬化 人脐静脉内皮细胞 

分 类 号：R543[医药卫生—心血管疾病]