藏药蕨麻多糖水解单糖的毛细管区带电泳分离研究  

Separation of Derivatized Carbohydrates of Potentilla anserina L.by Capillary Zone Electrophoresis

作　　者：夏莲[1,2,3] 孙志伟[2,3] 白新伟[1] 索有瑞[2] 尤进茂[1,2] 

机构地区：[1]生命有机分析重点实验室,曲阜师范大学化学与化工学院,山东曲阜273165 [2]中国科学院西北高原生物研究所,青海西宁810001 [3]中国科学院研究生院,北京100049

出　　处：《分析科学学报》2010年第1期25-30,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.20075016)

摘　　要：以1-萘基-3-甲基-5-吡唑啉酮(NMP)为柱前衍生试剂,探讨了毛细管区带电泳模式下对藏药蕨麻多糖水解液中单糖的分离条件。实验采用58.5 cm×50μm i.d.毛细管(有效长度50 cm),55 mmol/L硼酸盐缓冲溶液(pH=9.46),柱温20℃,分离电压22 kV,进样10 s。该法不加任何添加剂,9种单糖可高效、快速基线分离,实现了对藏药蕨麻多糖水解液中单糖的分离和定量分析,结果令人满意。A simple and rapid method was developed for the separation of derivatized carbohydrates of Potentilla anserina L. using 1-naphthyl-3-methyl-5-pyrazolone （NMP） as derivatization reagent by capillary zone electrophoresis. The detection was performed with diode array at 254 nm. A 58.5 cm× 50 μm i. d. （50 cm effective length） untreated fused-silica capillary was used. The optimum conditions are as follows：55 mmol/L borate buffer （pH=9. 46）, voltage 22 kV, column temperature at 20℃ and detection at 254 nm. The samples were atmospherically introduced with an injection time of 10 s. The results indicate that 9 NMP-derivatized carbohydrates achieve baseline resolution within 16 min under the proposed conditions. The Potentilla anserina L. sample was also analyzed under the optimum conditions.

关 键 词：多糖 蕨麻 1-萘基-3-甲基-5-吡唑啉酮 毛细管区带电泳 

分 类 号：O657.8[理学—分析化学]