天胡荽类药材及其混淆品HPLC指纹图谱鉴别研究  

Identification of Hydrocotyle sibthorpioides and its adulterants by HPLC fingerprint

作　　者：许士娜[1] 王祥培[1] 孙宜春[1] 靳凤云[1] 马艳妮[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中成药》2010年第2期173-176,共4页Chinese Traditional Patent Medicine

基　　金：国家中医药管理局"西部"重点学科建设资助项目(200414)

摘　　要：目的:建立天胡荽类药材HPLC指纹图谱的鉴别方法。方法:以Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱为分析柱,流动相为乙腈-0.1%磷酸水,采用梯度洗脱,流速1.0 mL/min,340 nm波长下检测。结果:天胡荽类药材的HPLC指纹图谱有明显差异。结论:该方法简便,重复性好,特征性强,可用于天胡荽类药材的鉴别。AIM ： To establish HPLC fingerprint for the identification of Hydrocotyle sibthorpioides and its adulterants. METHODS： The chromatographic seperation was performed on a Diamonsil C18 （250 mm×4.6mm,5μm） with a mobile phase consisting of acetonitrile-0.1% phosphoric acid, gradient eluent, at the flow rate of 1.0 mL/min. The UV detection was set at 340 nm. RESULTS： There was an apparent difference in fingerprint between Hydrocotyle sibthorpioides and its adulterants. CONCLUSION： The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Hydrocotyle sibthorpioides and its adulterants.

关 键 词：天胡荽 混淆品 HPLC 指纹图谱 鉴别 

分 类 号：R284.1[医药卫生—中药学]