HPLC测定清开灵颗粒中黄芩苷、栀子苷及胆酸、猪去氧胆酸的含量  

Determination of four active components in Qingkailing Granules by HPLC

作　　者：韩杰[1] 

机构地区：[1]北京市海淀区药品检验所,北京100083

出　　处：《中成药》2010年第2期227-231,共5页Chinese Traditional Patent Medicine

摘　　要：目的:用高效液相色谱法同时测定清开灵颗粒(胆酸、猪去氧胆酸、黄芩苷、栀子、水牛角、金银花、板蓝根、珍珠母)中黄芩苷、栀子苷2种苷类成分的含量;胆酸、猪去氧胆酸2种胆酸类成分的含量,为制定该制剂质量标准中含量测定方法及限度提供依据。方法:测定黄芩苷和栀子苷用kromasilTM C18分析柱(4.6 mm×150 mm,5μm),流动相:乙腈-水(10∶90),检测波长:238 nm,测定胆酸和猪去氧胆酸用kromasilTMC18分析柱(4.6 mm×250 mm,5μm),流动相:乙腈-水-磷酸(35∶65∶0.1),检测波长:192 nm。结果:该方法回收率黄芩苷:99.30%(RSD=0.2%),栀子苷:99.50%(RSD=0.4%),猪去氧胆酸:99.04%(RSD=0.2%),胆酸:99.06%(RSD=0.4%)。结论:结果表明该方法准确可靠,重现性好,结果稳定。AIM： To establish a method for determining baicalin, jasminoidin, cholalic acid and hyodeoxycholic acid in Qingkailing Granules（ chololic acid, hyodeoxycholic acid, baicalin, Fructus Gardeniae, Cornu Bubali, Flos Longicerae Japonicae, Radix Isatidis, Concha Margaritifera）. METHODS ： Part 1 （ determination of baicalin and jasminoidin） ：The HPLC method was carried out on kromasilTMC18 column（4. 6 mm × 150 mm,5 μm） with acetonitrile-water（ 10： 90）as mobile phase, gradient elution; the UV detection wavelength was at 238 nm. Part 2 （determination of cholalic acid and hyodeoxycholic acid） ：The HPLC method was carried out on kromasilWMCls column（4.6 mm× 150 Mm,5 μm） with aeetonitrile-water-phosphonic acid（35 ： 65 ： 0.1 ） as mobile phase,gradient elution ;the UV detection wavelength was at 192 nm. RESULTS： The average recoveries were 99.30% with RSD of 0.2% for baicalin; 99.50% with RSD of 0.4% for jasminoidin; 99.04% with RSD of 0.2% for hyodeoxycholic acid ; 99.06% with RSD of 0.4% for cholalic acid ; respectively. CONCLUSION ： The assay demonstrats that the method is simple, it has the adequate accuracy and selectivity to quantify the four active components in Qingkailing Granules.

关 键 词：HPLC 清开灵颗粒 黄芩苷 栀子苷 胆酸 猪去氧胆酸 

分 类 号：R927.2[医药卫生—药学]