高效液相色谱法测定复方葛酮颗粒中葛根素含量  

Determination of Puerarin in Compound Getong Granules by HPLC

作　　者：杨冬梅[1] 杨世友[2] 

机构地区：[1]安徽医学高等专科学校附属医院,安徽合肥230022 [2]安徽省药物研究所,安徽合肥230022

出　　处：《中国药业》2010年第5期19-20,共2页China Pharmaceuticals

基　　金：安徽省教育厅自然科学研究项目;项目编号:2006KJ380B

摘　　要：目的建立测定复方葛酮颗粒中葛根素含量的高效液相色谱(HPLC)法。方法以WatersC18柱(250mm×4.6mm,5μm)为色谱柱,流速1.0mL/min,检测波长250nm,甲醇-水-醋酸(25∶75∶1)为流动相。结果葛根素进样量的线性范围为0.048～1.92μg(r=0.9999,n=6),平均回收率为99.12%,RSD为1.62%(n=6)。结论HPLC法简便、准确,重现性好,可用于复方葛酮颗粒的质量控制。Objective To determine the content of puerarin in Compound Getong Granules by HPLC. Methods The HPLC method was used with the Waters C18 column （250 mm ×4.6 mm, 5 μm）. The mobile phase was methanol-water- glacial acetic acid （25：75：1）. The detection wavelength was at 250 nm and the flow rate was 1.0 mL/min. Results Puerarin had the good linear relationship in the concentrations between 0. 048 - 1.92μg（r=0. 999 9, n =6） with the average recovery rate of 99. 12% （RSD = 1.62% ）. Conclusion The method is simple, accurate, reliable and high sensitive, which can be regarded as the quality control method of Compound Getong Granules.

关 键 词：复方葛酮颗粒 葛根素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]