人类肠道病毒B组73、75和97型山东地方株的鉴定及其基因特征分析  

Identification and Genetic Characterization of Human Enterovirus Type 73,75,and 97 Strains of Specie B Isolated in Shandong Province

作　　者：陶泽新[1] 王海岩[1] 徐爱强[1,2] 宋立志[1] 李岩[1] 刘桂芳[1] 刘尧[1] 樊庆莹[2] 

机构地区：[1]山东省疾病预防控制中心,济南250014 [2]山东大学公共卫生学院,济南250012

出　　处：《病毒学报》2010年第1期16-19,共4页Chinese Journal of Virology

基　　金：山东省医药卫生重点项目计划课题(课题号:2007HZ090)

摘　　要：本研究对3例急性弛缓性麻痹患者标本的阳性病毒分离物进行了分子生物学定型。按照文献报道的方法,用扩增人类肠道病毒(Human Enterovirus,HEV)VP1完整编码区通用引物008-013、011-012进行逆转录-聚合酶链反应,反应产物纯化后进行序列测定。所得序列通过BLAST程序进行基因定型,显示3株病毒分别为HEV B组中新型病毒HEV73、HEV75和HEV97。将获得的序列与GenBank数据库中已有的各型病毒株VP1基因序列进行同源性分析并构建系统发生树,结果显示与各自的原型株相比有较大的变异,提示本次发现的HEV B组73、75、97型山东地方株与各型原型株相比具有不同的基因特征,这是在国内首次发现HEV97。Molecular typing was conducted for three human enteroviruses （HEV） isolated from acute flaccid paralysis （AFP） cases in Shandong province, China. RNAs from virus supernatants were extracted and complete VP1 genes were amplified by RT-PCR and sequenced. Genotypes of these isolates were identified as HEV type 73, 75 and 97, respectively by BLAST program. Homology and phylogenetic tree analyses were performed. Sequence analysis of VP1 gene showed significant variation compared with prototype strains. This study presents the genetic characteristics of HEV 73, 75 and 97 of specie 13 in Shandong Province, and the first report of HEV97 in China.

关 键 词：新型肠道病毒 急性弛缓性麻痹 分子进化 基因特征 

分 类 号：R373.2[医药卫生—病原生物学]