猪戊肝病毒存在准种现象  

Quasispecies Investigation on Swine Hepatitis E Viruses

作　　者：张西营[1] 朱瑞良[1] 朱于敏[2,3] 董世娟[2,3] 于瑞嵩[2,3] 沈世缘 王振勇[1] 李震[2,3] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]上海市农业科学院畜牧兽医研究所,上海201106 [3]上海市农业遗传育种重点实验室,上海201106

出　　处：《病毒学报》2010年第1期40-44,共5页Chinese Journal of Virology

基　　金：上海市科委2007年度重大科技攻关项目(07dz29508);上海市自然科学基金项目(200920832)的资助

摘　　要：为研究猪戊肝病毒准种存在情况,采用逆转录-巢式聚合酶链反应法（RT-nested-PCR）对四株猪戊肝病毒（Hepatitis E virus）第2可读框（ORF2）部分序列进行PCR扩增,将产物克隆后,每个毒株分别随机挑取20个阳性克隆测序,进行DNA序列分析。结果显示4株HEV不同克隆间的ORF2核苷酸序列同源性分别为96.8%-99.7%、98.8%-99.7%、98.8%-99.7%和100%,有变异的克隆核苷酸序列与上海株（SAAS-JDY5）同源性为96.8%-100%。由此证实感染戊肝病毒的猪个体内存在HEV准种。The objective of current study was to investigate the quasispecies of hepatitis E virus in swine. The partial ORF2 region of HEV envelope gene from four swine HEV strains was amplified by RT-nested polymerase chain reaction （RT-nPCR）. After cloning and transformation of PCR products, 20 positive clones of each HEV isolate were subject to sequencing and DNA analysis. The homology among the different clones of each isolates was 96.8%-99.7%, 98.8%-99.7%, 98.8%-99.7% and 100%, respectively, while there was 96.8%-100% sequence identity at the nucleotide level compared with HEV strains isolated in Shanghai（SAAS-JDY5）. This study confirmed that there existed quasispecies of HEV in swine.

关 键 词：猪 戊型肝炎病毒 变异 准种 

分 类 号：R373.9[医药卫生—病原生物学]