荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv.XushuNo.18)染色体  

FISH analysis of chromosomes of sweet potato(Ipomoea batatas cv.Xushu No.18)

作　　者：汤佳立[1,2,3] 戚大石[1,2] 张俞[1,4] 刘慧娟[1] 孙健英[1] 曹清河[3] 马代夫[3] 李宗芸[1,2] 

机构地区：[1]徐州师范大学生命科学学院,徐州221116 [2]徐州师范大学,江苏省药用植物生物技术重点实验室,徐州221116 [3]中国农业科学院甘薯研究所徐州甘薯研究中心,徐州221121 [4]北京师范大学生命科学学院,北京100875

出　　处：《遗传》2010年第2期177-182,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30800698);徐州市科技计划项目(编号:XM09B036);江苏省高等学校大学生实践创新训练计划项目(编号:09SSJCX07);江苏省环洪泽湖生态农业生物技术重点实验室开放基金项目(编号:HZHL0811)资助

摘　　要：为了解栽培种甘薯(徐薯18,Ipomoea batatas cv.XushuNo.18)的染色体结构,文章利用45SrDNA荧光原位杂交、自身基因组荧光原位杂交和银染技术对栽培种甘薯进行分子细胞遗传学研究。银染结果显示,徐薯18间期核有6对、8对和9对银染点;45SrDNA荧光原位杂交结果显示,徐薯18染色体上有8对或9对强弱不一的45SrDNA信号;自身基因组荧光原位杂交结果表明,所有染色体的全长分布强烈而密集的杂交信号,着丝粒区、近着丝粒区和端粒区有增强的信号带。In order to understand the chromosome structure of sweet potato （Ipomoea batatas cv. Xushu 18）, molecular cytogenetic analyses were carried out on I. batatas. by using 45S rDNA fluorescence in situ hybridization （45S rDNA-FISH）, self genomic in situ hybridization （self-GISH）, and silver staining techniques. Twelve, sixteen, and eighteen regions were silver stained in the interphase nucleus of I. batatas. The results of FISH analysis demonstrated 16 or 18 signals with different intensity on chromosomes of I. batatas. Self-GISH analysis showed that the intensive signals on I. batatas mitotic chromosomes were distributed along the chromosomes. However, the signals located in centromeric, subcentromeric, and telomeric regions were stronger and denser than those in other regions.

关 键 词：甘薯 银染 45SrDNA 荧光原位杂交 自身基因组原位杂交 

分 类 号：S531[农业科学—作物学]