煤工尘肺患者血清中基质金属蛋白酶-9及其抑制因子水平的变化  

Changes of serum matrix metaslloproteinases-9 and tissue inhibitors of metaslloproteinases-9 in coal workers＇ pneumoconiosis

作　　者：邹吉敏[1] 袁宝军[1] 王冬梅[1] 李超[1] 吴俊艳[1] 

机构地区：[1]华北煤炭医学院附属开滦医院检验科,唐山063000

出　　处：《中华劳动卫生职业病杂志》2010年第1期21-24,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨煤工尘肺患者血清中基质金属蛋白酶（MMP．9）及基质金属蛋白酶抑制剂（TIMP．9）水平的变化及意义。方法采用双抗体夹心ELISA法检测188例煤工尘肺（煤肺组53例，煤矽肺组67例，矽肺组68例）、57例0’期（0＋期组）、64例接尘对照组和50例健康对照组血清中MMP-9及TIMP-9含量；分析血清中MMP．9和TIMP-9水平在各煤工尘肺组及各对照组间的变化，以及二者的相关性；并根据煤工尘肺期别、是否有并发症以及接尘年限等因素进行分析。结果煤肺组血清中MMP-9水平为17．16ng／ml、煤矽肺组为15．14ng／ml，矽肺组为17．50ng／ml，与健康对照组比较，差异均有统计学意义（P〈O．05）与接尘对照组、0＋期组比较，均呈降低趋势，其中矽肺组及煤矽肺组差异有统计学意义（P〈0．05）。3组煤工尘肺患者血清中MMP-9水平比较，差异无统计学意义（P〉O．05）；煤肺组、煤矽肺组和矽肺组血清中TIMP。9水平分别为（330．00±108．42），（312．04±120．09），（366．81±135．50）ng／ml，均低于健康对照组，其中矽肺组和煤肺组血清中TIMP-9水平较接尘对照组、0’组明显增高，差异有统计学意义（P〈0．05），3组煤工尘肺组间比较，矽肺组血清中TIMP．9水平较煤矽肺组升高，差异有统计学意义（P〈0．05）。3组煤工尘肺患者血清中MMP-9和TIMP．9水平按不同的疾病分期、是否有并发症以及接尘年限进行分析，差异均无统计学意义（P〉0．05）；矽肺组血清中TIMP-9水平与年龄呈正相关（r=0．249，P〈0．05），血清中MMP-9和TIMP-9水平在接尘对照组中呈正相关（r=0．294，P〈0．05），其他组中未发现相关性。结论血清中MMP-9与TIMP-9水平变化与粉尘对机体的损害程度有关，MMP-9与TIMP-9共同参与了煤工尘肺的病理生理过程；动态观察0期及健康煤矿工人血清中MMP-9和TIMP-9水平，可为�Objective To explore the changes and significance of matrix metaslloproteinases （MMP） -9 and tissue inhibitors of metaslloproteinases（TIMP）-9 in serum of patients with coal workers＇ pneumoconio- sis（CWP）. Methods Serum MMP-9 and TIMP-9 were determined by sandwich ELISA in 188 cases of coal workers＇ pneumoconiosis （including 53 cases of coal pneumoconiosis, 67 cases of coal-silica pneumoconiosis and 68 cases of silicosis）, 57 cases of 0＋ miner （group 0＋）, 64 cases of heahhy miner （dust exposed control） and 50 healthy people （healthy control）, and were further evaluated according to stage, complication, and the duration of exposure to dust in CWP group. Results The median of serum MMP-9 in coal pneumoconiosis, coal-silica pneumoconiosis and silicosis were 17.16, 15.14 and 17.50 ng/ml respectively. The levels of serum MMP-9 in silicosis and coal-silica pneumoconiosis were lower than that in dust exposed control and 0＇ group （P〈0.05）, and there were no differences among 3 groups of CWP. The levels of serum TIMP-9 in coal pneu- moconiosis, coal-silica pneumoeoniosis and silicosis were （330.00±108.42）, （312.04±120.09） and （366.81± 135.50） ng/ml respectively, and compared with dust exposed control and 0＇ group, it increased in silicosis and coal pneumoeoniosis significantly （P〈0.05）. The levels of serum TIMP-9 in silicosis were higher significantly than that of coal-silica pneumoconiosis （P〈0.05）. No significant difference was observed in the levels of serum MMP-9 and TIMP-9 in CWP according to CWP stage, complications and the duration of exposure to dust （P〉 0.05 ）. There was a positive correlation between age and the concentration of TIMP-9 in silicosis （r=0.249, P〈 0.05）. A positive correlation was shown between the levels of serum MMP-9 and TIMP-9 only in dust exposed control （r=0.294,P〈0.05）. Conclusions The levels of serum MMP-9 and TIMP-9 are associated with the harmful effect to miner resulted from dust. The apparent distu

关 键 词：矽肺 基质金属蛋白酶 基质金属蛋白酶抑制因子 

分 类 号：R686[医药卫生—骨科学]