7.0T Mn^(2+)增强MRI追踪大鼠神经传导束  

Tracing rat nerve tracts with 7.0T Manganese-enhanced MRI

作　　者：文颂[1] 高歌军[1] 杨涛[1] 戴峰[1] 谭中宝[1] 

机构地区：[1]东南大学附属中大医院放射科,江苏南京210009

出　　处：《中国医学影像技术》2010年第1期29-32,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30770625);东南大学自然科学基金预研项目(9290001451)

摘　　要：目的探讨7.0T Mn2+增强MRI(MEMRI)追踪活体大鼠神经传导束的作用。方法SD大鼠9只,立体定位下向右侧运动皮层内注射1mol/L MnCl2溶液0.4μl,分别于注药前、注药后24h、48h、72h、7d行7.0T micro-MR扫描,观察皮质脊髓束及相关神经传导束的走行。结果MnCl2皮质内注射后分时间段进行MR扫描可以完整地显示活体大鼠皮质脊髓束从运动皮层、丘脑、大脑脚、脑桥的走行区结构,24~48h显示最佳,部分Mn2+通过胼胝体到达对侧运动皮层功能区。结论7.0T Mn2+增强MR能够清晰、动态地显示大鼠皮层内神经传导通路,Mn2+增强后的皮质脊髓束的解剖定位与Paxinos等大鼠解剖图谱定位一致;MEMRI能够显示两侧大脑半球间的联系,对活体研究大脑功能及损伤后脑的神经可塑性有重要的作用。Objective To investigate the effect of manganese-enhanced MRI（MEMRI）at 7.0T for tracing nerve tracts in rat brain in vivo.Methods With brain stereotactic apparatus,0.4 μl Mncl 2 with aqueous solution of 1 mol/L was injected into the right somatosensory cortex of 9 SD rats.MR scan was performed for tracing corticospinal tracts and other coherent nerve tracts pre-,and 24,48,72 h,7 days post-injection with 7.0T micro-MRI system,respectively.Results Corticospinal tracts were showed in intact after Mn ^2＋ administration from somatosensory cortex,thalamus,cerebral peduncle to pons at the time point of 24,48,72 h and 7 days,while the best tdisplaying was achieved at 24—48 h after Mn 2＋ administration.Simultaneously a small quantity of Mn ^2＋ reached the opposite somatosensory cortex through the corpus callosum.Conclusion MEMRI for tracing rat nerve tracts can be showed clearly with 7.0T MRI.The location of manganese-enhanced corticospinal tracts in agreement with the rat brain atlas in stereotaxic is in agreement with that Paxinos＇ published.MEMRI can display the relationship between the two sides of hemisphere,and may play an important role in investigating the brain function and nerve plasticity after nerve injury in vivo.

关 键 词：锰 磁共振成像 锥体束 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]