微囊藻毒素LR抑制小鼠淋巴细胞免疫功能的体外研究  

Suppression of lymphocyte immune function by MC-LR in vitro

作　　者：陈华[1] 林祥吉[1,2] 汪家梨[1] 储建忠[1,3] 

机构地区：[1]福建医科大学公共卫生学院,福州350004 [2]深圳市西乡卫生监督所,深圳518102 [3]福建省肿瘤医院,福州350014

出　　处：《癌变．畸变．突变》2010年第1期48-51,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：省科技三项费资助项目（K02070）

摘　　要：目的：探讨微囊藻毒素LR（microcystin LR,MC-LR）抑制小鼠淋巴细胞免疫功能的可能机制。方法：分离小鼠脾淋巴细胞,分别用含MC-LR为0、1、3、5、10μg/ml的培养液,染毒脾淋巴细胞,MTT法检测MC-LR对小鼠T、B淋巴细胞增殖的影响;ELISA法测定淋巴细胞IL-2的含量;FCM法检测淋巴细胞凋亡率。结果：各MC-LR剂量组淋巴细胞的增殖指数均下降;1μg/mlMC-LR刺激淋巴细胞分泌IL-2,而5、10μg/mlMC-LR组却抑制淋巴细胞分泌IL-2;10μg/mlMC-LR组淋巴细胞凋亡率明显升高（P均〈0.05）。结论：体外培养条件下,MC-LR能明显抑制小鼠脾淋巴细胞增殖,降低淋巴细胞因子IL-2的含量并诱导淋巴细胞凋亡。OBJECTIVE：To explore the suppression mechanism of MC-LR on lymphocyte immune function in mice.METHODS：Mice splenic lymphocytes were treated with MC-LR at the dose of 0,1,3,5,10 μg/ml in vitro.The T and B lymphocyte proliferations were examined by MTT assay.The production of IL-2 in cells was measured by ELISA and the apoptosis rate of lymphocytes was deteceted by FCM.RESULTS：The lymphoproliferation was suppressed by MC-LR at all doses.The production of IL-2 was increased at 1 μg/ml but decreased after treatment with MC-LR at 5 and 10 μg/ml.The rate of lymphocyte apoptosis was increased at 10 μg/ml in vitro.CONCLUSION：MC-LR could decrease the production of IL-2 of mouse lymphocyte but increase its apoptosis in vitro.MC-LR could markedly inhibit mice lymphocyte proliferation in vitro.

关 键 词：微囊藻毒素LR 淋巴细胞 IL-2 免疫功能 

分 类 号：R123[医药卫生—环境卫生学]