五味子提取物对CCl_4中毒大鼠肝超微结构的保护作用  

Protective effect of schisandrins extract on liver ultrastructure in rats with CCl_4 poisoning

作　　者：蔡绍先[1] 陈玮莹[2] 吴健谊[2] 张艳美[2] 蔡尔慧[2] 刘涛[2] 宾向玲[2] 温博贵[2] 

机构地区：[1]汕头大学医学院解剖学教研室 [2]汕头大学医学院生物化学教研室,广东汕头515041

出　　处：《癌变．畸变．突变》2010年第1期52-54,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省中医药局资助项目(20061060137;2007156);广东省汕头市重点科技计划项目(200682;200776)

摘　　要：目的:探讨五味子提取物对CCl4中毒大鼠肝细胞超微结构的保护作用。方法:Wistar大鼠25只,随机分为对照组、CCl4中毒24h组及10d组、五味子24h组及10d组,共5组,每组5只。CCl4中毒组大鼠用生理盐水(0.5ml/100g)灌胃3d,对照组和治疗组大鼠用五味子提取液(0.5ml/100g)灌胃3d后,中毒组和五味子组大鼠按0.2ml/100g体质量用量经腹腔一次性注射200ml/LCCl4致毒,对照组大鼠腹腔注射同体积植物油。然后,五味子组每天按0.5ml/100g体质量用量给予五味子提取液灌胃,而对照组、中毒组以等量生理盐水灌胃。不同时间点处死大鼠,透射电镜下观察肝脏超微结构的改变。结果:CCl4中毒24h组大鼠肝组织超微结构发生改变,表现为胞质基质疏松、空泡化;肝细胞线粒体肿胀,嵴断裂或消失;内质网呈片层状;核膜皱缩、断裂,染色质边集于核膜下,CCl4中毒10d组肝细胞核形态仍呈明显异型性。与CCl4中毒组大鼠相比较,五味子组大鼠肝细胞超微结构的病理性改变减轻。结论:五味子提取物对CCl中毒大鼠肝细胞的超微结构具有保护作用。OBJECTIVE：To explore the protective effect of Schisandrins extract on liver ultrastructure in rats with CCl4 poisoning.METHODS：Twenty five Wistar rats were randomly divided into the control group,the CCl4 poisoned 24 h and 10 d groups,the Schisandrins 24 h and 10 d groups,five groups with 5 rats in each group.The rats of the CCl4 poisoned and the Schisandrins groups were given one peritoneal injection of CCl4（200 ml/L in vegetable oil） at a dosage of 0.2 ml/100 g body weight,the rats of the control group were given the same volume of vegetable oil.Then Schisandrins extract（0.5 ml/100 g body weight）were given orally to rats of the Schisandrins group daily,while saline was given to rats of the poisoned and the control groups.The rats were sacrificed at different time points.The morphologic changes of the hepatocytes were examined by transmission electron microscopy.RESULTS：The ultrastructure of hepatocytes of rats were changed in the CCl4 poisoned 24 h group,the cytoplasm of hepatocytes was loosed and vacuolated,mitochondria were swollen,with cristae breakdown or loss,and the endoplasmic reticulum appeared lamellar.The nuclear membrane was shrunken and ruptured,and the nuclear chromatin condensed and marginated.The hepatocytic nuclei showed obvious atypia in rats of the CCl4 10 d group.By comparing with that of the CCl4 group,the ultrastructure of hepatocytes in rats of the schisandrins group was less severely damaged.CONCLUSION：The use of Schisandrins extract in the treatment of CCl4 poisoned rats exerted a protective effect on liver ultrastructure.

关 键 词：四氯化碳 大鼠 五味子 肝 超微结构 

分 类 号：R285.2[医药卫生—中药学] Q954.6[医药卫生—中医学]