1,8-桉油精对卵白蛋白致哮喘豚鼠的气道高反应性和炎症的抑制作用  

Inhibitory effects of 1,8-cineol on ovalbumin-induced lung inflammation and airway hyperresponsiveness in asthmatic guinea pigs

作　　者：徐巧萍[1] 王砚[1] 唐法娣[1] 夏锦芳[1] 刘君波[1] 赵小京[1] 卞如濂[1] 

机构地区：[1]浙江大学医学院呼吸药物研究室,浙江杭州310058

出　　处：《中国药理学与毒理学杂志》2010年第1期35-43,共9页Chinese Journal of Pharmacology and Toxicology

基　　金：浙江省中医药重点项目研究计划资助项目(2008ZA014)~~

摘　　要：目的探讨1,8-桉油精(1,8-cineol)对哮喘豚鼠肺功能的改善作用及其机制。方法豚鼠第0天和第7天ip给予0.5ml含卵白蛋白(OVA)20μg的氢氧化铝凝胶致敏,28d后OVA攻击制备哮喘模型。观察豚鼠OVA攻击后1h,1,8-桉油精10,30和100mg.kg-1对豚鼠吸入OVA后1,2,3,4,10,20和30min时气道阻力(Raw)和肺顺应性(Cdyn)变化及支气管肺泡灌洗液(BALF)中白细胞数和细胞分类的影响,并测量豚鼠肺组织中嗜酸细胞阳离子蛋白(ECP)、白细胞介素4(IL-4)、IL-8和肿瘤坏死因子α(TNF-α)的含量。观察豚鼠OVA攻击后17h再吸入乙酰甲胆碱(MCh)后,1,8-桉油精10,30和100mg.kg-1对Raw和Cdyn及BALF中白细胞数和细胞分类的影响,并测量豚鼠肺组织中ECP,IL-4,IL-8和TNF-α的含量。结果与正常对照组比较,豚鼠OVA攻击后1h,在4min时模型组Raw达到高峰;与模型组比较,1,8-桉油精30和100mg.kg-1明显抑制Raw增加(P<0.05);在3min时模型组Cdyn达到高峰,与模型组比较,1,8-桉油精10,30和100mg.kg-1均能明显抑制Cdyn降低(P<0.05);模型组豚鼠肺组织ECP,IL-4和TNF-α含量明显高于正常对照组(P<0.05);1,8-桉油精100mg.kg-1组ECP,IL-4和TNF-α含量均明显低于模型组(P<0.05),模型组与正常对照组豚鼠肺组织IL-8含量无明显差异。与模型组比较,1,8-桉油精100mg.kg-1能明显减少BALF中白细胞数和嗜酸性粒细胞比例(P<0.05)。致敏豚鼠OVA攻击17h后,模型组豚鼠Raw与正常对照组比较显著升高(P<0.05),模型组豚鼠Cdyn与正常对照组比较有显著性差异(P<0.01),1,8-桉油精100mg.kg-1对MCh引起的Raw的增加有明显的抑制作用,1,8-桉油精10,30和100mg.kg-1对MCh引起的Cdyn降低有明显的改善作用;与模型组相比,1,8-桉油精100mg.kg-1能明显减少BALF中白细胞数和中性粒细胞比例,降低肺组织ECP,IL-8和TNF-α含量(P<0.01);模型组与正常对照组豚鼠肺组织IL-4含量无明显差异;1,8-桉油精30mg.kg-1也能降低哮喘豚鼠肺组织中ECP和TNF-α含量(P<0.01)。结�OBJECTIVE To investigate the effects of 1,8-cineol on lung functions and mechanism in asthmatic guinea pigs.METHODS The guinea pig model was performed by intraperitoneal injection of the 0.5 ml Al（OH）3 gel containing OVA 20 μg.The guinea pigs were constructed by immunization of intraperitoneal injection on the 0 day and the 7th day,and the experiment was performed on the 28th day.The effect of 1,8-cineol 10,30 and 100 ml·kg^-1 on the airway resistance（Raw） and dynamic lung compliance（Cdyn） of asthmatic guinea pigs 1 h after challenge of OVA.The changes in leukocyte and different kinds of leukocyte in bronchoalveolar lavage fluid（BALF） after the challenge of OVA have been studied.The levels of eosinophil cationic protein（ECP）,interleukin（IL）-4,IL-8 and tumor necrosis factor（TNF）-α in lungs of guinea pigs were determined using enzyme-linked immunosorbent assay（ELISA）.The changes in Raw and Cdyn of asthmatic guinea pigs were investigated 17 h after challenge of OVA and inhalated methacholine（MCh）.The changes in leukocyte and different kinds of leukocyte in BALF after the challenge of OVA have been studied.The levels of ECP,IL-4,IL-8 and TNF-α in lungs of guinea pigs were determined using ELISA.RESULTS 1,8-Cineol inhibited increase in Raw and decrease in Cdyn from 1 to 30 min after challenge of OVA in model group.The levels of ECP,IL-4 and TNF-α in asthmatic model group were higher than those in normal control group（P〈0.05）.The levels of ECP,IL-4 and TNF-α of 1,8-cineol 100 mg·kg^-1 group were significantly lower than those in asthmatic model group（P〈0.01）.The level of IL-8 of asthmatic model group didn＇t have any significant difference from that of control group.1,8-Cineol 100 mg·kg-1 could significantly decrease the numbers of leukocyte and the percent of eosinophils in BALF.Seventeen hours after challenge of OVA,Raw and Cdyn of asthmatic model group were higher than these of control group（P〈0.05,P〈0.01）;1,8-cineol 100 mg·kg^-1 significantly inhibit

关 键 词：1 8-桉油精 哮喘 气道高反应性 炎症 细胞因子 

分 类 号：R285[医药卫生—中药学] R974[医药卫生—中医学]