不同剂量地塞米松对兔海水淹溺性肺水肿细胞因子变化的影响  

Effects of dexamethasone on cytokine levels of rabbits with pulmonary edema induced by seawater drowning

作　　者：张兵华 赵亚玲 费晋秀 巨敏 胡平 王娟 

机构地区：[1]解放军323医院干三科,陕西西安710054 [2]解放军323医院检验科,陕西西安710054

出　　处：《西北国防医学杂志》2010年第1期4-6,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：兰州军区医药卫生科研基金资助项目(LXH-2008008)

摘　　要：目的:探讨地塞米松对兔海水淹溺性肺水肿(PE-SWD)模型的治疗作用及其对血清IL-8、TNF-α及IL-10的影响。方法:40只家兔随机分为对照组(CG,10只)和不同剂量(0.05 mg/kg、0.2 mg/kg、1.0 mg/kg)地塞米松治疗组(DG1、DG2、DG3,各10只)。用酶联免疫法(ELISA)测定淹溺前及淹溺后30、120 min血清中IL-8、TNF-α及IL-10的含量,并进行动脉血气分析。结果:与CG组比较,DG2、DG3组PaO2升高(P<0.05),IL-8、TNF-α含量显著下降(P<0.01),IL-10含量明显增加(P<0.01);DG2组与DG3组比较,PaO2、IL-8、TNF-α、IL-10无明显变化(P>0.05)。结论:较大剂量地塞米松能减轻肺损伤,其机制可能与抑制IL-8、TNF-α的释放,增加IL-10的生成有关。但增加剂量(1 mg/kg)并不能增加治疗效果。Objective：To investigate the therapeutical effect of dexamethasone on rabbits with pulmonary edema induced by seawater drowning and detect the changes of serum IL-8,TNF-α and IL-10 levels.Methods： Forty rabbits were randomly divided into 4 groups： control group（CG）,dexamethasone group（0.05 mg/kg as DG1,0.2 mg/kg as DG2,1 mg/kg as DG3）.The levels of serum IL-8,TNF-α,and IL-10 were examined by ELISA.Arterial blood gas was analyzed.Results：Compared with CG,the PaO2 in DG2 and DG3 was increased（P0.05）,the serum IL-8 and TNF-α levels were decreased（P0.01）,the serum IL-10 levels was increased（P0.01）.There was no significant difference between DG2 and DG3（P0.05）.Conclusion：Dexamethasone at comparatively high dose attenuates lung injury after seawater drowning,which may be related to the IL-8,TNF-α reduction and IL-10 increase.However,higher dose（1 mg/kg） can not improve the treatment effect.

关 键 词：地塞米松 海水淹溺 肺水肿 家兔 

分 类 号：R649.3[医药卫生—外科学]