不同产地了哥王药材中总黄酮的含量测定  

Determination of the Content of Total Flavonoids in Wikstroemia Indica from Different Producing Areas

作　　者：熊友香[1] 尤志勉[2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]温州市中医院,浙江温州325000

出　　处：《中华中医药学刊》2010年第2期358-359,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20050821)

摘　　要：目的:对不同产地了哥王药材中总黄酮的含量测定并进行比较。方法:样品制备后加5%亚硝酸钠溶液1mL,放置6min后,加10%硝酸铝溶液1mL,放置6min后,加4%氢氧化钠溶液10mL,15min后,甲醇定容至刻度线,摇匀,在波长λ=510nm下测定吸光度,以芦丁做对照。结果:江西、广西、湖南3个产地的了哥王药材中的总黄酮平均含量分别为2.19%、2.52%、1.72%,广西产4批药材中含量相差较小。结论:可见不同产地,因不同的生长环境、温度、湿度等因素对了哥王中总黄酮的含量影响较大。Objective：To Study the methodology of the content of total flavonoids in Wikstroemia indica from different producing areas.Methods： Add 1mL sodium sulfite liquid of 5% concentration to the sample after it is prepared,add 1mL aluminium nitrate liquid of 10% concentration 6 minutes later,add 10mL sodium hydroxide liquid of 4% concentration 6 minutes later,keep the carbinol to the constant volume of the tick mark 15 minutes later and shake it up.Mensurate the absorbency under the wavelength of λ=510nm and compare it with the eldrin.Results：The average content of total flavonoids in Wikstroemia indica from the three producing area of Jiangxi,Guangxi and Hunan are 2.19%,2.52% and 1.72%.The difference of content in four batchs of medicinal materials from Gaungxi Province are comparatively small.Conclusion： Due to different producing areas,the growing environments,temperature and humidity have comparatively big impacts on the content of the total flavonoids in Wikstroemia indica.

关 键 词：了哥王 不同产地 黄酮含量 

分 类 号：R285[医药卫生—中药学]