电针对局灶性脑缺血再灌注大鼠大脑皮层HSP70蛋白的影响  

The Effect of Focal Cerebral Ischemia Reperfusion and Electro-acupuncturing Bilateral HSP70 Expression in Sprague-Dawley (SD) Rat Brain Tissue

作　　者：王伟[1] 闫也[2] 

机构地区：[1]盱眙县中医院内科,江苏盱眙211700 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2010年第2期360-362,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅资助项目(2004SD275)

摘　　要：目的:探讨电针对局灶性脑缺血再灌注大鼠大脑皮层HSP70蛋白表达的影响,进一步验证电针抗脑缺血损伤的机理与HSP70的关系。方法:采用线栓法制备大鼠大脑中动脉闭塞模型(MCAO),随机分组,经免疫组化(SABC法)、He染色、神经功能缺陷评分,观察大鼠缺血侧大脑皮层HSP70蛋白表达阳性细胞数,组织学损伤分级及神经功能缺陷评分的变化。结果:脑缺血再灌注电针组较对照组HSP70阳性细胞数多,组织学损伤分级低,神经功能缺陷评分低,且具有显著性差异(P<0.01)。结论:电针可以提高局灶性脑缺血再灌注大鼠大脑皮层HSP70蛋白的表达,针刺治疗脑缺血的机制可能与HSP70蛋白增加有关,为临床针刺治疗脑缺血疾病提供又一新的理论依据。The effect of focal cerebral ischemia reperfusion and electro-acupuncturing bilateral ＂bai hui＂ and＂ren zhong＂ point on HSP70 expression in Sprague-Dawley（SD） rat brain tissue was evaluated by the SABC immunohistochemistry method and HE staining.Compared with a control group,HSP70 expression increased markedly in I90min/1h,I90min/R6h 190min/R12h groups（P0.01）.Compared with the I90min /R6h group,it was observed that electro-acupuncture could further increase HSP70 expression（P0.05）.The results suggest that the neuroprotective mechanism of electro-acupuncture on focal cerebral ischemia reperfusion may be related to the upregulation of HSP70 expression.

关 键 词：电针 局灶性脑缺血 再灌注损伤 HSP70 

分 类 号：R249[医药卫生—中医临床基础]