RP-HPLC法测定乌骨藤药材中Tenacissoside B的含量  

Determination of Tenacissoside B in the Stems of Marsdenia Tenacissima by RP-HPLC

作　　者：马备娟 李晓誉[2] 叶益萍[2] 

机构地区：[1]金华人民医院药剂科,浙江金华321000 [2]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中华中医药学刊》2010年第2期362-363,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技计划资助项目(F200513028)

摘　　要：目的:建立乌骨藤药材中Tenacissoside B的含量测定方法。方法:采用高效液相色谱法测定乌骨藤中Tenacissoside B的含量,色谱柱使用Diamonsil C18(250mm×4.6mm,5μm);流动相为甲醇∶水(78∶22);检测波长为217nm;流速1mL/min;柱温30℃。结果:Tenacissoside B在进样量1.01～10.1μg范围内线性关系良好,回归方程为:y=432.41x-13.14,R2=0.9998;平均回收率为98.30%,RSD为1.78%(n=6)。结论:该方法简便,灵敏,准确,重现性好,可用于乌骨藤药材的含量测定和质量控制。Objective： To establish a method for determination of the content of Tenacissoside B in the roots of Marsdenia tenacissima.Methods：The analysis was carried out by RP-HPLC on Diamonsil C18（250mm×4.6mm,5 μm）,Methanol∶water（78∶22） as mobile phase;wave length was 217 nm;flow rate was 1.0mL/min;column temperature was 30℃.Results： The liner range of Tenacissoside B was 1.01～10.1μg.Regression equation was： y=432.41x-13.14,R2= 0.9998.The average recovery was 98.30% with RSD=1.78%（n=6）.Conclusion： The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis for the stems of Marsdenia tenacissima.

关 键 词：乌骨藤 Tenacissoside B 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]