中心组合试验设计和响应面分析优化柑桔皮中柚皮苷提取工艺  

The Optimization of Extracting Naringin from Orange Peel by the Central Composite Design Experiments and Response Surface Methodology

作　　者：白云凤[1] 袁辉[1,2] 薛胜霞 

机构地区：[1]温州医学院药学院,浙江温州320035 [2]浙江科技学院浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023 [3]杭州余杭区第三人民医院,浙江杭州311115

出　　处：《中华中医药学刊》2010年第2期372-375,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y307415);浙江科技学院人才启动基金资助项目(0101103501)

摘　　要：目的:探讨超声波辅助提取柑桔皮中柚皮苷的优化工艺条件。方法:通过单因素试验考察了提取温度、提取时间、料液比、乙醇体积分数及提取次数5个因素对柚皮苷提取率的影响,并在此基础上通过中心组合试验设计和响应面分析优化柑桔皮中柚皮苷提取工艺。结果:在料液比为1∶30、超声时间30min、提取温度58℃、乙醇体积分数60%,提取2次的实验条件下,柑桔中柚皮苷具有最高的提取率3.38%。结论:与传统的提取方法相比,超声波提取法不仅节省时间,而且提取效率高,是一种工艺简单、高效、节能的新型提取工艺。Objiective：In this paper a simple way-ultrasonic wave technique was used to extract the naringin from the orange peel.Methods：The effects of extracting temperature,time,number,solid-liquid ratio and ethanol concentration factors on the naringin extraction rate were investi-gated by single-factor experiments.Based on the above work,the central composite design experiments and response surface methodology（RSM） was used in the optimization of technological parameters.Results：The results showed that the rate of raw material and solvent 1∶30,extracting time 30 min,ethanol concentration 60%,temperature 58℃ and extracting 2 times,were optimal and the extraction rate was 3.38%.Conclusion：Compared to traditional extraction methods,ultrasonic wave had the advantages of reducing extraction time,simple procedure and improving yield.

关 键 词：中心组合试验设计 响应面分析 柚皮苷 

分 类 号：R284.2[医药卫生—中药学]