HPLC波长切换法测定莪术残油中莪术二酮莪术醇和吉马酮的含量  

Determination of Curdione Curcumol and Germacrone of Residual Zedoary oil by HPLC

作　　者：孙艳涛[1] 刘姝畅[1] 张振秋[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中华中医药学刊》2010年第2期391-392,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:建立用HPLC波长切换法同时测定莪术残油中莪术二酮、莪术醇和吉马酮含量的测定方法。方法:采用Kromasil 5μm C18(4.6mm×150mm);流动相为乙腈-1%磷酸溶液;流速1.0 mL.min-1;检测波长213 nm,236nm;柱温35℃。结果:莪术二酮在0.1215～0.6076μg范围内线性关系良好,平均回收率为100.0%,RSD为2.4%;莪术醇在0.5760～2.880μg范围内线性关系良好,平均回收率为99.9%,RSD为2.1%;吉马酮在2.014～10.07μg范围内线性关系良好,平均回收率为100.3%,RSD为2.3%。结论:不同批号的莪术残油中3种成分的含量差异不显著。Objective：To establish the method of determine the content of Curdione,Curcumol and Germacrone in residual Zedoary oil by HPLC.Methods： Kromasil 5μm C18（4.6mm×150mm）;mobile phase of acetonitrile-1% phosphoric acid;flow rate of 1.0 mL ·min-1;detection wavelength 213 nm,236nm;Column temperature： 35℃.Results： Curdione 0.1215～0.6076μg at the scope of a good linear relationship,the average recovery 100.0%,RSD 2.4%;Curcumol 0.5760～2.880μg at the scope of a good linear relationship,the average recovery 99.9%,RSD 2.1%;Germacrone at the scope of 2.014～10.07μg a good linear relationship,the average recovery 100.3%,RSD 2.3%.Conclusion： Different batch of residual zedoary oil composition of three kinds of content not significantly.

关 键 词：莪术油 莪术二酮 莪术醇 吉马酮 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]