NGF与CNTF联合治疗糖尿病大鼠胃肠神经病变的实验研究  

Combined Effect of rhCNTF and NGF on Intestine Function Neuropath in Diabetes Mellitus Rat

作　　者：刘庆山[1,2] 张莉[1] 李韶菁[1] 杨志宏[1] 崔箭[3] 杜冠华[1] 

机构地区：[1]中国医学科学院-北京协和医学院药物研究所,北京100050 [2]中央民族大学中国少数民族传统医学研究院,北京100081 [3]中央(民族大学中国少数民族传统医学研究院,北京100081

出　　处：《中国药学杂志》2010年第2期111-114,共4页Chinese Pharmaceutical Journal

基　　金：国家高新技术(863)重大专项项目(2004AA273782);国家自然科学基金项目资助(30973959)

摘　　要：目的为了研究重组人睫状神经营养因子(rhCNTF)与神经生长因子(NGF)联合给药对糖尿病大鼠胃肠神经功能紊乱的作用。方法采用链脲佐菌素(streptozocin,STZ)诱发Ⅰ型糖尿病大鼠所致胃肠神经功能紊乱模型,动物随机分为正常组、模型组、NGF(0.02 mg.kg-1,im)组、rhCNTF(0.1 mg.kg-1,im)组、NGF和rhCNTF联合用药组,连续给药85 d。观察各组大鼠血糖、胃肠慢波电位、小肠壁内神经蛋白含量、胃肠推进功能以及胃肠对乙酰胆碱(Ach)、阿托品敏感性。结果NGF和rh-CNTF联合用药和单独用药对糖尿病大鼠血糖均无明显降低作用,十二指肠对Ach、阿托品的敏感性也未见明显改变,但均可改善糖尿病大鼠胃肠慢波电位,提高胃肠推进功能,同时增加了胃肠壁内神经蛋白含量,联合用药效果优于单独给药。结论NGF与RhCNTF联合用药具有较好的抗糖尿病导致的胃肠神经功能紊乱作用,其机制可能与他们对内脏植物神经的神经营养和神经保护作用有关,且具有一定的协同作用。OBJECTIVE To investigate the eombined effect of recombinant human ciliary neurotrophic iactor （rhCNTF） and nerve growth factor （NGF） on gastrointestinal neurosis in diabetic rats. METHODS Diabetic rat models were created by STZ. Rats were randomly divided into normal group, model group, NGF group （0. 02 mg.kg^-1 , im） , rRhCNTF group （0. 1 mg.kg^-1 , ira） , NGF ＋ RhCNTF group（0. 02 and 0. 1 mg.kg^-1 , respectively, ira）. Rats were continuously administered for 85 d. The blood sugar, slow wave in stomach intestine, S100 in intestine, peristalsis, sensitivity to Aeh and artropine in intestine were detected ＇after administrating for 85 days. RESULTS The blood glucose was not changed compared with to the model group, and the response of jejunam to Ach and atropine was not changed too. The frequency of slow wave of jejunum the content of S100 and the fnnctions of tire intestine were improved. CONCLUSION Tire function of intestine in diabetes rat can be improved by administering rhCNTF, and the important mechanism is the nerve protective effects of rhCNTF.

关 键 词：神经生长因子 重组人睫状神经营养因子 糖尿病 胃肠功能 神经病变 

分 类 号：R965[医药卫生—药理学]