HPLC-UV定量分析清热灵颗粒中的5个成分  

Simultaneous Determination of Five Compounds in Qingreling Granules by HPLC-UV

作　　者：徐金钟[1] 邢丽红[1] 王书芳[1] 瞿海斌[1] 

机构地区：[1]浙江大学药物信息学研究所,杭州310058

出　　处：《中国药学杂志》2010年第2期147-149,共3页Chinese Pharmaceutical Journal

基　　金：教育部新世纪人才支持计划资助(NCET-06-0515)

摘　　要：目的建立一种HPLC-UV同时测定清热灵颗粒中5种成分(黄芩苷、连翘苷、汉黄芩苷、黄芩素和汉黄芩素)的含量。方法采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),以0.05%甲酸-水和0.05%甲酸-乙腈为流动相,梯度洗脱。流速为1.0 mL.min-1,检测波长为274 nm。结果5个成分的峰面积与浓度的线性关系良好(r2≥0.999 7);加样回收率(RSD≤3.77%)、精密度(RSD≤0.65%)、重现性(RSD≤2.78%)和稳定性(RSD≤3.19%)均符合有关要求。结论该方法简单,准确可靠,重现性好,可用于清热灵颗粒质量的控制和评价。OBJECTIVE To establish a HPLC-UV method for the simuhaneous determination of five compounds including baica- lin, phillyrin, wogonoside, baicalein and wogonin in Qingreling granules. METHODS The separation was performed on an Agilent Zorbax SB-C18 column （4. 6 mm×250 mm, 5 μm） with gradient elution. The mobile phases consisted of 0. 05% formic acid-acetonitril and 0. 05% formic acid-water at a flow rate of 1.0 mL.min^-1. The detective wavelength was 274 nm. RESULTS 5 compounds showed good linearity （ r^2 ≥10. 999 7 ） in the ranges of tested concentration. The recoveries （ RSD ≤ 3.77% ）, reproducibility （ RSD ≤ 0. 65% ）, precision（ RSD ≤2. 78% ） and stability （ RSD ≤3.19% ） for 5 compounds were satisfactory. CONCLUSION The method is simple, accurate and reliable. It may be used for the quality control of Qingreling granules.

关 键 词：清热灵颗粒 高效液相色谱法 黄芩苷 连翘苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R917[医药卫生—药物分析学]