胰岛素样生长因子-Ⅰ对雌性大鼠尿道括约肌损伤后修复的影响  

Influence of periurethral injection of insulin-like growth factor- Ⅰ on the expression of IGF- Ⅰ and IGF- Ⅱ mRNA following urethral sphincter muscle injury in female rats

作　　者：陈园[1] 杜广辉[1] 蔡丹[1] 胡卫锋[1] 章慧平[1] 谢冲[1] 陈忠[1] 袁晓奕[1] 杨为民[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《中华泌尿外科杂志》2010年第2期119-122,共4页Chinese Journal of Urology

基　　金：国家自然科学基金项目资助（30772290）

摘　　要：目的探讨胰岛素样生长因子I（IGF—Ⅰ）对雌性大鼠尿道括约肌损伤后修复的影响。方法雌性未育SD大鼠55只分3组，治疗组（n=25）采用阴道扩张模拟产伤方法建立尿道括约肌损伤模型，尿道中段注射IGF-Ⅰ1．0μg（浓度0．1μg／μl）；生理盐水对照组（n=25）尿道括约肌损伤模型建立后中段尿道注射无菌生理盐水10μl；正常对照组（n=5）无损伤不做任何处理。RT—PCR法检测治疗组、生理盐水对照组损伤后2、4、6、8、14d时及正常对照组大鼠尿道括约肌组织IGF-Ⅰ和ⅡmRNA的相对表达量，比较治疗组和生理盐水对照组尿道括约肌损伤后不同时间IGF—Ⅰ和HmRNA的表达差异。结果尿道括约肌组织伤后2、4、6、8、14d，生理盐水对照组大鼠IGF-Ⅰ mRNA表达龋分别为未测出、0．58±0．15、1．73±0．31、2．30±0．29、0．46±0．06，治疗组分别为0．69±0．21、1．45±0．17、2．25±0．45、2．90±0．49、1．92±0．31，与生理盐水对照组比较第4、8、14天表达差异有统计学意义（P〈0．05）；生理盐水对照组尿道括约肌组织伤后4、6、8d IGF-Ⅱ mRNA表达量分别为0．42±0．14、1．51±0．59、1．31±1．04，治疗组4、6、8、14d表达量分别为1．04±0．23、1．94±0．29、1．75±0．41、0．81±0．15，与生理盐水对照组比较第4天差异有统计学意义（P〈0．01）。正常对照组尿道组织无IGF—Ⅰ、ⅡmRNA表达。结论局部注射IGF—Ⅰ能促进尿道括约肌损伤局部内源性IGF-Ⅰ和ⅡmRNA表达，IGF—Ⅰ对尿道括约肌损伤所致压力性尿失禁可能具有潜在的治疗作用。Objective To investigate the effect of periurethrat injection of insulin-like growth factor（IGF）- Ⅰ on the expression of IGF- Ⅰ and IGF- Ⅱ mRNA during regeneration period following urethral sphincter muscle injury in female rats. Methods Model of urethral sphincter muscle injury was made in female virgin SD rats （n= 50） by intravaginal balloon inflation. Then the rats were divided randomly into treatment group （n= 25） and control group （n=25）, treatment group acceptedperi urethral injection of 1.0 μg human IGF- Ⅰ to the middle urethral muscle, control group accepted nor mal saline injection. Five rats in each group were sacrificed at 2, 4, 6, 8, 14 day respectively and the whole urethra specimens were processed for RT-PCR to detect the expression of IGF- Ⅰ , ⅡmRNA. A normal control group （n= 5） was set without intravaginal balloon inflation and injection. Results The expression of IGF- Ⅰ mRNA in control group increased at day 4, 6, 8, 14, the IGF-Ⅰ/β-actin ratios were 0.58±0. 15, 1. 73±0.31, 2. 30±0. 29, 0.46±0. 06. The expression of IGF- Ⅰ mRNA in treatment group increased at all time points, as 0.69±0.21, 1.45±0.17, 2.25±0.45, 2.90±0.49, 1.92±0.31. The difference was significant on day 4, 14（P〈0.01）, and clay 8 （P〈0.05） compared with the control group. The expression of IGF Ⅱ mRNA in control group increased at day 4, 6, 8, as 0.42±0. 14, 1.51±0.59, 1. 31±1.04. The expression of IGF-Ⅱ mRNA in treatment group increased at day 4, 6, 8, 14, as 1.04±0.23, 1.94±0.29, 1.75±0.41, 0.81±0. 15. The significant difference was noted on day 4（P〈0.01）compared with the control group. No expression of IGF-Ⅰ and Ⅱ mRNA in the normal control group. Conclusions The expression of endogenous IGF- Ⅰ and IGF- Ⅱ mRNA was up-regulated by periurethral injection of IGF- Ⅰ during regeneration period following urethral sphincter muscle injury in female rat. Our findings suggest that IGF-Ⅰ facilitates the re generation of the urethral muscles and may pl

关 键 词：胰岛素样生长因子 尿道括约肌 损伤 压力性尿失禁 

分 类 号：R694.5[医药卫生—泌尿科学]