北极狐GHR基因cDNA的克隆及序列分析  

cDNA cloning and sequence analysis of growth hormone receptor (GHR) from Arctic fox

作　　者：杜智恒[1] 宁方勇[1] 杨春山[1] 白秀娟[1] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030

出　　处：《兽类学报》2010年第1期65-73,共9页Acta Theriologica Sinica

摘　　要：本文根据狗(AF133835)的GHR基因cDNA编码全序列设计了三对引物,利用RT-PCR方法克隆出北极狐GHR基因编码区全长cDNA序列(GenBank accession No.EU304325)。结果表明,北极狐GHR的ORF为1917bp,编码638个氨基酸的前体蛋白,由18个氨基酸的信号肽和620个氨基酸的成熟肽组成。通过同源性比较发现北极狐与狗的同源性最高,达到98%。另外,利用邻接法(NJ法)构建的分子系统进化树聚类结果表明,北极狐与狗先聚为一类,该聚类结果与传统的物种进化关系基本一致。另外,通过氨基酸对位序列比较发现,北极狐GHR在氨基酸序列上存在明显的特异性,如45和451位分别为A和E,而其它物种均分别为T(大鼠为K)和A(牛羊为V,鼠为T)。In the current study, the gene encoding growth hormone receptor （GHR） was cloned by RT-PCR from Arctic fox （Alopex lagopus） according to dog GHR gene （AF133835）. Sequence analysis revealed that the open reading frame（ORF）of Arctic fox GHR is 1 917 bp, which encodes a 638-amino acid precursor containing a signal peptide of 18 amino acids and a mature peptide of 620 Amino acids. Arctic fox GHR gene has highest similarity（98%） with dog GHR.The molecular phylogenetic tree was constructed by the NJ method based on GHR gene sequences. The result showed the Arctic fox and the dog were clustered into one branch, this result is consistent with traditional classification. In addition, Arctic fox GHR has its own characterization in its amino acid sequence compared to other mammal GHR sequences. For example, its amino acid residues at position 45 and 451 are A and E, respectively, while for others species the corresponding amino acid residues are T（K for rat ）and A（V for cattle and sheep and T for mouse）.

关 键 词：北极狐 GHR基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]