高效液相色谱-串联质谱法检测蔬菜水果中65种农药残留方法研究  

Study on Determination of Multi-Residues for 65 Pesticides in Vegetables and Fruits Using Liquid Chromatography Coupled with Mass Spectrometry

作　　者：刘宝峰[1] 刘罡一[2] 马又娥[1] 余琛[2] 

机构地区：[1]上海市农药研究所,上海200032 [2]上海市徐汇区中心医院中心实验室,上海200031

出　　处：《科技通报》2010年第1期93-99,共7页Bulletin of Science and Technology

基　　金：上海市科学技术委员会应用技术开发专项资金资助

摘　　要：建立了一种高效液相色谱-串联质谱法（LC／MS／MS）同时测定蔬菜、水果中65种农药多残留的方法，并应用于样品中农药残留的定性定量检测。样品以乙腈提取，经石墨碳／氨基串联柱固相萃取，采用C18柱分离．以含0．02％甲酸乙腈-0．02％甲酸水溶液为流动相梯度洗脱，多反应监测（MRM）模式下，以保留时间和质荷比对分离出的组分予以定性确证，外标法峰面积定量。65种农药在一定的范围内线性关系良好（r〉0．995）．样品中最低检出浓度为0．02～20μg／kg，样品的平均加标回收率为70％～120％。方法简便、快速、灵敏，适用于蔬菜、水果中这65种农药的残留分析。A muhi-residue method for the determination of 65 pesticides in vegetables and fruits by means of LC/MS/MS is presented. The pesticides were extracted by acetonitrile, enriched and cleaned up by SPE. Chromatographic separation was performed on a C18 column using a gradient elution of aeetonitrile-water containing 0.02% formic acid.The average recoveries ranged from 70%-120%,and the limit of detection （LOD） was 0.02-20 μg/kg. The method is simple,rapid, accurate and suitable for simultaneous determination of these pesticides in vegetables and fruits.

关 键 词：农药 多残留 液相色谱-串联质谱 

分 类 号：S481.8[农业科学—农药学]