间接荧光抗体试验诊断泡球蚴病的研究  

STUDY ON INDIRECT FLUORESCENT ANTIBODY TECHNIC IN DIAGNOSIS OF HUMAN MULTILOCULAR ECHINOCOCCOSIS

作　　者：王兰珍[1] 张京元[1] 崔凯[2] 王浩 朱克安 孙忠贵 刘家祥[4] 李明[4] 

机构地区：[1]宁夏医学院寄生虫学教研室 [2]宁夏医学院附属医院外科 [3]宁夏固原地区人民医院外科 [4]宁夏西吉县人民医院外科

出　　处：《宁夏医学杂志》1990年第1期1-3,共3页Ningxia Medical Journal

摘　　要：本文分别采用人肝细粒棘球蚴的囊壁切片和原头蚴石蜡切片两种抗原对52例泡球蚴病人、56例非泡球蚴病人和108例健康人进行了间接荧光抗体试验(IFA)。结果,对泡球蚴病人血清的敏感性均为100％,与酶联金葡菌A蛋白酶免疫吸附试验(PPA—ELISA)者相当;特异性分别为98.2％和98.8％,也与PPA—ELISA者相当,无显著差异。Indirect fluorescent antibody technic ([FA) on paraffin section antigen from cyst wall/ saolex of human hepatic Echinococcus granulosus hydatid cyst was Performed on the sera of 52 patients with multilocular echinococ-cosis,56 Patients not caused by echi-nococcosis> and 108 healthy adults. The optimal dilutions of sera and fluorescent antibody were selected to be 1:20 and 1:10 respectively. Fluorescence intensity or more than was considered as positive.Thesensi-tivities of the above two antigens we~ reall 100%, their specif icities were 98.2%and 98.8% respectively, showing no significant difference.Diagnosticv-alues of IFA and peroxidase protein A enzyme-linked immunosorbent assay (P -PAELISA) were compared on the se-raof 30 patients with multilocularech-inococcosis, 25 patients not caused by echinococcosis, and 51 healthy adults,statistically, they showed no significant differences

关 键 词：泡球蚴病 荧光抗体试验 诊断 IFA 

分 类 号：R532.320.4[医药卫生—内科学]