维生素C对甲醛胚胎发育毒性抑制作用  

Inhibitive effect of vitamin C on embryonic development toxicity induced by formaldehyde in mice

作　　者：郑翔[1] 曹智丽[1] 曹霞飞[1] 郑宇[1] 王超[1] 曾怀才[1] 龙鼎新[1] 让蔚清[1] 

机构地区：[1]南华大学公共卫生学院环境医学与放射卫生研究所,湖南衡阳421001

出　　处：《中国公共卫生》2010年第2期180-182,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30572191)

摘　　要：目的探讨维生素C(VC)对甲醛诱导胎鼠发育毒性的影响。方法应用体外全胚胎培养模型,观察100μg/mL VC对终浓度为9,27,81μmol/L甲醛诱导昆明种小鼠胚胎发育毒性的影响。结果与对照组比较,9μmol/L甲醛可使胚胎头长、颅臀长、谷胱甘肽(GSH)、丙二醛(MDA)含量分别升高到(1.66±0.21),(3.48±0.40)mm,(47.70±2.12)mg/(g.prot),(4.27±0.62)nmol/(mg.prot),差异有统计学意义(P<0.05);27μmol/L甲醛使胚胎脏层卵黄囊(VYS)直径、头长、颅臀长、体节数、GSH含量分别下降到(4.35±0.65),(1.48±0.30),(3.19±0.43)mm,(25.2±1.2)对,(40.25±4.38)mg/(g.prot),MDA含量升高到(6.29±0.47)nmol/(mg.prot),与对照组比较,差异有统计学意义(P<0.05);VC能明显改善上述指标,但与对照组比较差异无统计学意义(P<0.01);VC不能明显改善81μmol/L甲醛诱导的毒性损伤。结论VC对一定剂量范围内的甲醛胚胎毒性具有明显抑制作用。Objective To observe the inhibitive effect of vitamin C（VC） on embryonic development toxicity induced by formaldehyde in mice. Methods Kunming mice embryos were incubated together with formaldehyde （ 9, 27, and 81 μmol/L） and VC（ 100 μg/mL） ,respectively, with whole embryo culture method. The development of mice embryos was then assessed. Results When the concentration of formaldehyde was 9μmol/L, the increases of head lengh （ 1.66 ± 0.21 mm）, cranial arm length （ 3.48 ± 0. 40mm ）, glutathione （GSH） （ 47.70 ± 2. 12mg/g prot ） and malonaldehyde （MDA） （4. 27 ±0. 62nmol/mg · prot） were significantly different（P 〈0. 05） from those of the control group. When the concentration was 27 μmol/L, the increases of embryo visceral yolk sac （VYS） diameter（ 4. 35 ± 0. 65mm）, head lengh （ 1. 48 ±0. 30 mm）, cranial arm length （ 3. 19 ± 0. 43mm ）, number of arthromera pairs （ 25. 2 ± 1.2 ） and GSH （40. 25 ±4. 38mg/g · prot） and the decrease of MDA（6. 29 ±0. 47nmol/mg· prot） were observed with significant differences （ P 〈 0. 05 ） compared with that of the control group. VC could improve the indexes but there was no significant difference between the control group and the VC group （P 〉 0. 05 ）. The toxic injury induced by formaldehyde （81 μmol/L） could not be improved with VC significantly. Conclusion VC inhibits embryonic development toxicity induced by certain dose of formaldehyde.

关 键 词：甲醛 维生素C 发育毒性 体外全胚胎培养 

分 类 号：R54[医药卫生—心血管疾病]