妊高征患者外周血B细胞体外分泌抗体能力的研究  

The Study on in Vitro Antibody Secretion of B Lymphocyte in Patient with Pregnancy Induced Hypertention

作　　者：郑英如[1] 陈竹钦[1] 章容[1] 颜建华 

机构地区：[1]重庆第三军医大学第三附属医院野战外科研究所

出　　处：《中华妇产科杂志》1998年第12期717-719,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金

摘　　要：目的研究妊高征患者Ｂ细胞的功能变化，从免疫学的角度探讨妊高征的发病机理。方法采用密度梯度离心法分离妊高征组（１５例）和正常妊娠组（１５例）的外周血Ｂ淋巴细胞，并在有或无美洲商陆有丝分裂原（ＰＷＭ）存在的情况下培养１０天后，收集上清液，采用酶联免疫吸附试验（ＥＬＩＳＡ）测定每１×１０６个Ｂ淋巴细胞所分泌的ＩｇＧ和ＩｇＭ的量。结果在无ＰＷＭ存在的情况下，妊高征组Ｂ淋巴细胞ＩｇＧ和ＩｇＭ的自发分泌量（１２０±６８ｎｇ，３３７±１１９ｎｇ）分别与正常妊娠组ＩｇＧ和ＩｇＭ的自发分泌量（１５４±８９ｎｇ，３７１±１３５ｎｇ）无显著差异；而在ＰＷＭ的刺激下，妊高征组Ｂ淋巴细胞分泌ＩｇＧ（９４７±５１８ｎｇ）和ＩｇＭ（２０２３±８７９ｎｇ）的量分别显著低于正常妊娠组分泌的ＩｇＧ（１３６７±５９８ｎｇ）和ＩｇＭ（２８４０±１０５７ｎｇ）的量（Ｐ＜０．０５）。结论妊高征患者Ｂ淋巴细胞针对有丝分裂原的刺激信号而产生应答反应的能力下降。Objective To study the immunologic etiopathology of pregnancy induced hypertention (PIH) in some extent by analyzing the antibody secretion of B lymphocytes of PIH patients. Methods The subjects were divided into two groups: PIH group ( n = 15) and normotensive control group ( n =15). Peripheral lymphocytes of the subjucts were separated by Ficoll Hypaque density centrifugation. The lymphocytes were cultured in RPMI 1640 medium with or without pokeweed mitogen (PWM) for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay (ELISA).The results of the two groups were analyzed by using student test. Results Both IgG and IgM secretion of B lymphocytes between PIH group and normtensive control group showed no difference in the absence of PWM(per 1×10 6 lymphocytes, for IgG ,120±68 ng vs 154±89 ng, P >0.05;for IgM, 337±119 ng vs 371±135 ng , P >0.05. Whereas the level of the two antibodies in PIH group was significantly decreased than that of the normotensive group in presence of PWM (for IgG,947±518 ng vs 1 367±598 ng, P <0.05; for IgM, 2 023± 879 ng vs 2 840±1 057 ng, P <0.05). Conclusion B lymphocyte of PIH patient has a decreased capacity of antibodies secreting in response to stimulation of PWM, which suggests that B lymphocyte of PIH patient may have functional deficiency.

关 键 词：B细胞 IgG IGM 妊娠高血压 综合征 

分 类 号：R714.246[医药卫生—妇产科学]