翠云草总黄酮对结肠癌HT-29细胞COX-2 mRNA表达的抑制作用  

Inhibiting Action of Total Flavones from Selaginella uncinata on COX-2 mRNA Expression in HT-29 Cells

作　　者：孙颖桢[1,2] 陈科力[1] 刘震[1] 

机构地区：[1]省部共建中药资源和中药复方教育部重点实验室湖北中医学院药学院,武汉430065 [2]紫琅职业技术学院生物技术系

出　　处：《中国药师》2010年第2期163-164,168,共3页China Pharmacist

基　　金：国家自然科学基金项目(编号:30470193)

摘　　要：目的:研究翠云草总黄酮对结肠癌HT-29细胞环氧化酶-2(COX-2)mRNA表达的影响。方法:以翠云草总黄酮作用于人结肠癌HT-29细胞,用倒置显微镜观察细胞形态学的变化;并设总黄酮高中低剂量组和阴性对照组共4个组别,采用逆转录聚合酶链反应(RT-PCR)法检测给药后癌细胞COX-2 mRNA表达的水平,用电泳凝胶成像自动分析系统检测各扩增带的产物含量。结果:与阴性对照组比较,翠云草总黄酮可以在mRNA水平上抑制COX-2的表达,且呈量效关系。结论:翠云草总黄酮能在mRNA水平上抑制COX-2,进而抑制COX-2蛋白质表达,这可能是翠云草抗肿瘤作用的机制之一。bjective ： To investigate the action of total flavones from Selaginella uncinata （ Desv. ） Spring on cyclooxygenase-2 （COX-2） mRNA expression in carcinoma of colon cell line HT-29 ,and explore related molecular mechanism thereby. Method： Total flavones from Selaginella uncinata was used on TH-29 cells and there were four groups including high-dose group, midst-dose group, low-dose group and negative control group. The morphological change of the cells was observed by inverted microscope. The expressions of COX-2 mRNA were measured using reverse transcription polymerase chain reaction （RT-PCR） method. Caraphoresis gel photo auto- analysis system was used to detect the products. Result： Comparing with the negative control group, the total flavonoids groups could significantly inhibit the expressions of the COX-2 mRNA level in a dose-dependent manner. Conclusion： It can be concluded that the mechanisms may be that the total flavonoids can inhibit COX-2 in mRNA level. Then it can inhibit COX-2 protein expression. It may be one of the anti-tumor mechanisms of Selaginella uncinata.

关 键 词：翠云草 HT-29 环氧化酶-2 逆转录聚合酶链反应法 

分 类 号：R285.5[医药卫生—中药学]