小鼠高脂血症造模方法的比较及优化  

Comparison and Optimization of the Method for Establishing the Hyperlipidemia Model in Mice

作　　者：吴娟[1] 柳冬月[1] 顾施健[1] 周志文[1] 宋成武[1] 胡雪峰[1] 但红[1] 方念伯[1] 余尚工[1] 

机构地区：[1]湖北中医学院中药资源与中药复方省部共建教育部重点实验室,武汉430065

出　　处：《中国药师》2010年第2期165-168,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:30873469)

摘　　要：目的:通过不同造模方法的比较来建立新的高脂血症造模方法。方法:对四组小鼠分别进行正常喂养、乳剂灌胃、高脂饲料喂养和在高脂饲料喂养的同时隔天乳剂灌胃。然后在第0,7,14,21天检测小鼠血压以及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)含量,并在实验结束时测定脏器系数。结果:乳剂+高脂组肝系数显著高于其他组;血压值在14 d后显著升高(P<0.01);TC含量和TG含量分别在7 d和14 d后显著升高(P<0.01);HDL-C含量在7 d后显著性降低(P<0.01);第21天与第14天相比,血压和血脂水平能够继续维持,并且乳剂+高脂组较乳剂灌胃组小鼠死亡率低。结论:本造模方法切实可行,在短期内效果明显。Objective： To develop a new method for establishment of hyperlipidemia animal model by comparing different methods. Method. Four groups of mice were given normal diet respectively,intragastric administration of fat emulsion once a day,high fat diet whole day and high fat diet whole day with given intragastric administration of fat emulsion every two days. The blood pressure and serum levels of TC, TG and HDL-C were measured at the first day, the seventh day,the fourteenth day and the twenty-first day. At the end of experimental period, the organ coeffcient was calculated. Result： Compared with other groups,the liver coefficient of the group with high fat diet whole day and intragastric administration of fat emulsion was statistically increased, the blood pressure level of this group were statistically increased after treatment of fourteen days （ P 〈 0. 01 ） ; The content of TC and TG statistically were increased after treatment of seven days and fourteen days, respectively （P 〈 0. 01 ） ;The content of HDL-C was decreased after treatment of seven days （P 〈0. 01 ） ;By comparison with the fourteenth day treatment,the blood pressure level and the high blood lipids level of the twenty-first day treatment stay steadily. The group with high fat diet whole day and intragastric administration of fat emulsion had low mice morality. Conclusion： The new method is practically workable and has a significant effect in a short time.

关 键 词：高脂血症 高血压 模型 

分 类 号：R965.1[医药卫生—药理学]