皱瘤海鞘水提物体外抗HSV-2活性实验研究  

Water Extract of Styela Plicata Inhibit HSV-2 Activity in Vitro

作　　者：周至品[1,2] 万新祥[1] 刘叔文[3] 

机构地区：[1]南方医科大学药剂研究中心,广州510315 [2]广西中医学院附属瑞康医院 [3]南方医科大学抗病毒研究中心

出　　处：《中国药师》2010年第2期176-178,共3页China Pharmacist

基　　金：广州市科技计划项目(2005J1-C0401)

摘　　要：目的:了解皱瘤海鞘水提物体外抗单纯疱疹病毒2型的活性。方法:采用HSV-2在Vero细胞上的增殖为模型,用细胞病变法和MTT法对皱瘤海鞘水提物体外抗HSV-2的药效活性进行评价。结果:皱瘤海鞘水提物CC_(50)值大于10 mg·ml^(-1),毒性低;对HSV-2具有很好抑制和杀伤作用,2 mg·ml^(-1)时对病毒的生物合成抑制蟀达到82.98%,对HSV-2的直接杀伤率为74.29%。对HSV-2的吸附阻断作用较小。结论:皱瘤海鞘水提物具有良好的抗HSV-2活性。Objective： To study the role of water extract of Styela plicata on inhibiting herpes simplex virus 2 （HSV-2） activity in vitro. Method： The cytopathic effect assay and MTT assay were applied to investigate Styela plicata anti-HSV-2 activity in vitro. Result： The CC50 value of water extract of Styela plicata was greater than 10 mg. ml - 1, toxicity was very low. It had a role of anti-HSV-2, the inhibitory rate was 82. 98% at 2 mg.m1-1 ,and kill rate was 74. 29%. It could not inhibit the adsorption of HSV-2. Conclusion： Water extract of Styela plicata has a good role of anti-HSV-2 activity in vitro.

关 键 词：皱瘤海鞘 水提物 单纯疱疹病毒2型 体外 

分 类 号：R285.5[医药卫生—中药学]