HPLC法测定少阳感冒颗粒中黄芩苷的含量  

Determination of Baicalin in Shaoyang Ganmao Granules by HPLC

作　　者：杨小月[1] 

机构地区：[1]怀化市药品检验所,湖南怀化418000

出　　处：《中国药师》2010年第2期228-229,共2页China Pharmacist

摘　　要：目的:建立HPLC法测定少阳感冒颗粒中黄芩苷的含量。方法:采用Diamonsil C_(18)柱(250 mm×4.6 mm,5μm);以甲醇-水-冰醋酸(50:50:1)为流动相,流速为1.0 ml·min^(-1);检测波长为274 nm。结果黄芩苷进样量在0.05～0.28μg范围内线性关系良好,r=0.999 7,平均加样回收率为98.0%,RSD为0.82%(n=6)。结论:方法简便易行、准确、重复性好,可用于少阳感冒颗粒的质量控制。Objective： To establish an HPLC method for the determination of baicalin in shaoyang ganmao granules. Method： A Diamonsil C18 column （250 mm ×4.6 mm,5 μm） was used,the methanol-water-glacial acetic acid （50： 50：1 ） as mobile phase,the flow rate was 1.0 ml.min-1 ;the detection wavelengh was 274 nm. Result： A good linear was obtained within the range of 0.05-0. 28 μg, r = 0. 999 7. the average recovery was 98. 0% （ RSD = 0. 82% , n = 6 ）. Conclusion： The mothod is simple, accurate and repeatable,it can be used for the quality control of shaoyang ganmao granules.

关 键 词：高效液相色谱 少阳感冒颗粒 黄芩苷 含量测定 

分 类 号：R927.2[医药卫生—药学]