IL-7对胸腺T细胞及树突状细胞体外分化发育的影响  

Interleukin-7 influences the development of thymic T lymphocytes and thymic dendritic cells

作　　者：许云云[1] 王泳[1] 李毅平[1] 李芳[1] 徐小霞[1] 朱守兵[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所,苏州215007

出　　处：《中国免疫学杂志》2010年第2期108-112,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30400395);国家教育部留学回国人员科研基金资助项目(K5120506)

摘　　要：目的:探讨IL-7对胸腺T细胞及胸腺树突状细胞分化的影响。方法:摘取15～16日龄胎鼠胸腺进行体外器官培养(胚胎胸腺器官培养-FTOC),分别将细胞因子IL-7和培养基滴加在胸腺小叶上,12天后收集不同条件下经FTOC培养获得的胸腺细胞,流式细胞仪检测细胞表面分子CD4、CD8、CD11c、B220、Ia等的表达,通过光学显微镜观察细胞形态,通过细胞计数检测细胞数目的变化。再将经FTOC培养获得的胸腺细胞和异源的T细胞进行混合淋巴细胞反应,通过MTT法检测T细胞的增殖情况。结果:细胞计数结果表明添加外源性IL-7组的胸腺细胞数目明显减少,流式细胞仪检测结果显示其中胸腺CD4-CD8-双阴性细胞及CD8+单阳性细胞比例有所增加,而CD4+CD8+双阳性细胞比例显著下降,CD4+单阳性细胞比例没有明显变化;此外,B细胞和树突状细胞、NK细胞数量均有不同程度的增加。结论:IL-7在胸腺T细胞及胸腺树突状细胞的分化发育中发挥重要的调节作用。Objective：To investigate the effect of IL-7 on differentiation of thymic T lymphocytes and dendritic cells＇ differentiation in vitro. Methods： Thymic lobes were apspectically removed from 15-day-old or 16-day-old mouse fetueses and were cultured by the way of fetal thymus organ cultures （FFOC） in vitro. Lobes were cultured in the dishes with or without Interleukin-7（ 1L-7）, then collected the cultured ceils respectively. The expression of CD4, CD8, CD11 c, B220, Ia, and CDI I b was detected by flow cytometry, and the morphology was observed under the ligt microscope. The proliferation of these cells was determined by cell counting. The cultured cells were used as stimulators for allogeneic T cells, whose proliferation were detected later by MTT method. Results： The result of cell counting indicated that the total amount of the thymocytes was decresed obviously after being cultured with IL-7, while the proportion of CD4 ＋ CD8 ＋ thymocyles decreased, the proportion of CI）4- CD8- , and CD4- CD8 ＋ thymocytes increased, and no much difference in the amount of CD4 ＋ CD8 - thylnocytes was found. In addition, the amount of B lymphocyles, natural killer cells, and dendritic cells increased in different degree. Conclusion： IL-7 participates in differentiation and development of thymic T lymphocytes and dendritic cells.

关 键 词：胚胎胸腺器官培养 IL-7 胸腺T细胞 胸腺树突状细胞 

分 类 号：R392.1[医药卫生—免疫学]