B细胞趋化因子对CVB3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响  

The influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1

作　　者：高志云[1] 揣侠[1] 蓝佳明[1] 刘贵霞[1] 李剑[1] 张永红[1] 王永祥[1] 

机构地区：[1]河北医科大学病原生物学教研室,石家庄050017

出　　处：《中国免疫学杂志》2010年第2期117-119,共3页Chinese Journal of Immunology

基　　金：河北省科学技术研究与发展计划项目(No.08276101D-93)

摘　　要：目的:探讨B细胞趋化因子(B-lymphocyte chemoattractant,BLC)对柯萨奇病毒B3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响。方法:将BALB/c小鼠随机分为4组,分别肌肉注射pcDNA3、pcDNA3/BLC、pcDNA3/C3d3-sVP1和含pcDNA3/BLC与pcDNA3/C3d3-sVP1的混合质粒,于免疫后不同时间检测小鼠血清的中和抗体滴度、特异性CTL杀伤活性;以LD50的CVB3病毒液感染已免疫小鼠,检测小鼠血中病毒滴度,观察存活情况以评价各种疫苗的免疫保护作用。结果:小鼠的中和抗体滴度随免疫次数增加而提高,混合组中和抗体滴度(42.17±1.43)和特异性CTL杀伤率(41.3%±3.51%)均明显高于pcDNA3/C3d3-sVP1组(P<0.05),而血中病毒滴度低于pcDNA3/C3d3-sVP1组;经致死量CVB3感染后,pcDNA3/BLC和pcDNA3/C3d3-sVP1混合免疫的小鼠生存率达44%,生存率高于其他各组。结论:BLC可显著提高C3d3-sVP1基因诱导的特异性免疫应答,增强基因疫苗对机体的免疫保护作用。Objective：To investigate the influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1. Methods： BALB/c mice were divided into 4 groups randomly, and injected intramuscularly with pcDNA3, pcDNA3/ BLC,pcDNA3/C3d3-sVP1 and the combination with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1. At a certain time, they were measured for the titers for neutralizing antibodies, specific CTL cytotoxic activity. The protective efficacy of DNA vaccinations was evaluated by titers of blood viruses and survival rate. Results： The titers for antibodies increased with the time of inoculation. More specifically, the antibody titers （42.17 ±1.43） and the specific CTL cytotoxic activity （41.3% ±3.51% ） of the mice in the combination group were remarkably stronger than in the mice with pcDNA3/C3d3-sVP1 （ P 〈0. 05）, but the virus titers of blood was lower. After lethal CVB3 challenge, the protection of mice from death in the combination group with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1 was 44%. Survival curves indicated that the survive state of combination group was better than others. Conclusion： BLC can strongly enhance the specific immunity induced by C3d3- sVP1.

关 键 词：B细胞趋化因子 补体3d 柯萨奇病毒B3 基因疫苗 

分 类 号：R373.2[医药卫生—病原生物学]