新型微波解冻装置解冻冰冻红细胞的方法学评价  

Methodological evaluation of new microwave thawing devices in thawing frozen red blood cells

作　　者：张三明[1] 周宏宇[1] 涂洪钢 石展鹰[1] 李春荣[1] 黄建强[1] 

机构地区：[1]解放军113医院,浙江宁波315040

出　　处：《中国临床新医学》2010年第2期109-111,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：南京军区2007年度医药科技创新资助项目

摘　　要：目的应用新型微波解冻装置解冻冰冻红细胞,检测质量控制指标,并对新型微波解冻装置和红细胞制备效果进行评价。方法应用新型微波解冻装置解冻以甘油为保护剂-80℃保存的红细胞,用不同浓度的洗涤液洗涤去除甘油,以红细胞回收率、游离血红蛋白含量、残余甘油含量、体外溶血试验和无菌试验为质量检测控制项目。结果新型微波解冻装置能快速冰冻红细胞,解冻后红细胞质量指标均达到《全血及成分质量要求》(GB18469-2001)的要求,在红细胞回收率方面有明显提高,并且缩短了制备时间。结论新型微波解冻装置能快速解冻冰冻红细胞,质量符合标准,为解决血液长期储存、保障供血和稀有血型患者输血及自身输血提供了新的方法和手段,特别在野战、自然灾害等特殊条件下急救能发挥作用,值得推广应用。Objective To use the new microwave thawing devices for thawing frozen red blood cells, test quality control indicators, and evaluate the new microwave thawing devices and the effects of red blood cell preparation. Methods The new microwave thawing devices were used for thawing the red blood cells preserved in glycerol - 80 ℃, the glycerol was removed with different concentrations of washing liquid, the red blood cell recovery, free hemoglobin, residual glycerol content, in vitro hemolysis test and the sterility test were used as for the quality inspection control project. Results The new microwave thawing devices can quickly thaw the frozen red blood cells, the quality indicators of thawed red blood cells were in accord with ＂whole blood and component quality requirements＂ （GB18469-2001）, the recovery rate of red blood cells was markedly improved and the preparation time was shortened. Conclusion The new microwave thawing devices can quickly thaw frozen red blood cells, the quality was in accord with standard. It provide new ways and means for the long-term storage of blood, guarantee of the blood supply, blood transfusion in patients with rare blood groups and blood transfusion itself, especially in first aid of the field, natural disasters and other special conditions it can play a role, it is worth popularizing.

关 键 词：新型微波解冻装置 微波水热法 冰冻红细胞 甘油 评价 

分 类 号：R457.1[医药卫生—治疗学]