转录因子AP-2β与SLP-2的蛋白相互作用  

The Interaction Between Transcription Factor AP-2β and SLP-2

作　　者：王义军[1] 徐甜[1] 胡翔[1] 向双林[1] 周建林[1] 张健[1] 韩梅[1] 

机构地区：[1]湖南师范大学生命科学学院,蛋白质化学与发育生物学教育部重点实验室,中国湖南长沙410081

出　　处：《生命科学研究》2010年第1期16-20,共5页Life Science Research

基　　金：国家973资助项目(2005CB522505);国家973资助项目(2006CB943506);湖南省科技厅资助项目(2007CK3052);湖南省教育厅资助项目(07c563)

摘　　要：AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀结合质谱鉴定的方法筛选与AP-2β相互作用的蛋白.结果筛选到Stomatin like protein2(SLP-2)蛋白可能与AP-2β相互作用.免疫共沉淀实验证实外源过表达和内源的SLP-2蛋白都能与AP-2β蛋白相互作用.而且,细胞免疫荧光实验发现外源表达的Myc-AP-2β蛋白与内源的SLP-2蛋白在MCF-7细胞的胞质中有共定位.此外,免疫印记实验结果表明过表达SLP-2能上调AP-2β的蛋白水平,这就表明SLP-2与AP-2β相互作用后,SLP-2可能使AP-2β蛋白更稳定.这些研究结果为进一步研究这两个基因的功能提供了新的切入点.AP-2β is an important member of transcription factor AP-2 family. AP-2 is a transcription factor implicated in mammalian development, cell proliferation, apoptosis and carcinogenesis. Recent studies have shown that interaction partners can modulate the transcriptional activity of AP-2 over the downstream targets. To identify potential AP-2β interacting partners, we screened AP-2β immunocomplexes by mass spectrometry and identified SLP-2 as a putative AP-2β interacting protein. It is demonstrated that both exogenously expressed and endogenous SLP-2 interacted with AP-2βin vivo by co-immunoprecipitation assays. In addition, indirect immunofluorescent analyses demonstrated that AP-2β and SLP-2 co-localized in the cytoplasm of the MCF-7 cell lines. Furthermore, over-expression of SLP-2 enhanced the protein expression level of AP-2β. Thus, through protein-protein interaction, SLP-2 can stabilize AP-2β. These results provided a new clue for further research on the AP-2β and SLP-2 function.

关 键 词：AP-2β 相互作用蛋白 SLP-2 免疫荧光分析 免疫共沉淀 

分 类 号：Q78[生物学—分子生物学]