Optimization of parental RNAi conditions for hunchback gene in Locusta migratoria manilensis （Meyen）  

作　　者：Zheng-Bo He Yue-Qing Cao Yu-Xian Xia 

机构地区：[1]Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing, China

出　　处：《Insect Science》2010年第1期1-6,共6页昆虫科学（英文版）

摘　　要：An investigation on the optimization of parental RNA interference （RNAi） conditions for hunchback （hb） gene in Locusta migratoria manilensis （Meyen） was conducted. Double stranded RNA （dsRNA） corresponding to hb gene was injected into haemocoel of female adults ofL. migratoria manilensis. Embryos developed from the eggs laid by the injected adults on the 7th day after eclosion showed observable effects of RNAi for hb. The silencing effect after delivery treatment of dsRNA for hb gene was maintained for more than 21 days. A significant decrease of hb transcripts was further confirmed by Northern blot analysis. The dose of dsRNA/insect at 2μg could trigger 96.9% RNAi effects, while silencing appeared to have no dependence on the size of dsRNA. Results suggest that parental RNAi could be employed to efficiently identify the developmental gene functions in L. migratoria manilensis.An investigation on the optimization of parental RNA interference （RNAi） conditions for hunchback （hb） gene in Locusta migratoria manilensis （Meyen） was conducted. Double stranded RNA （dsRNA） corresponding to hb gene was injected into haemocoel of female adults ofL. migratoria manilensis. Embryos developed from the eggs laid by the injected adults on the 7th day after eclosion showed observable effects of RNAi for hb. The silencing effect after delivery treatment of dsRNA for hb gene was maintained for more than 21 days. A significant decrease of hb transcripts was further confirmed by Northern blot analysis. The dose of dsRNA/insect at 2μg could trigger 96.9% RNAi effects, while silencing appeared to have no dependence on the size of dsRNA. Results suggest that parental RNAi could be employed to efficiently identify the developmental gene functions in L. migratoria manilensis.

关 键 词：HUNCHBACK Locusta migratoria manilensis parental RNA interference 

分 类 号：Q522[生物学—生物化学] S433.2[农业科学—农业昆虫与害虫防治]