多重PCR和单重PCR在流感病毒快速检测中的比较与应用  

COMPARISON AND APPLICATION OF MULTIPLEX PCR AND SINGLE PCR TECHNIQUE FOR THE RAPID DETECTION METHOD OF INFLUENZA VIRUS

作　　者：季亚勇[1] 沙丹[1] 马广源[1] 尤凤兴[1] 居丽雯[2] 施强[2] 吴家林[1] 凌霞[1] 肖勇[1] 

机构地区：[1]无锡市疾病预防控制中心,无锡214023 [2]上海复旦大学公共卫生院

出　　处：《现代预防医学》2010年第4期758-760,共3页Modern Preventive Medicine

基　　金：卫生部科研基金(WKJ2006-2-10)

摘　　要：[目的]建立快速检测H1、H3亚型和乙型流感病毒的多重PCR技术,并与单重PCR技术进行比较与应用。[方法]根据H1、H3亚型和乙型流感病毒设计引物,进行多重PCR扩增及电泳检测,在快速检测中与单重PCR进行比较与应用。[结果]该多重PCR技术能在4h内同时检测3种型别的流感病毒,时间短于单重PCR技术,特异性和灵敏度相似。[结论]本研究建立多重PCR技术可以准确检测流感病毒,不仅灵敏度高、特异性好,而且可以得到快速的检测结果。[Objective] To establish rapid detection technology of multiplex PCR to isolate H1, H3 subtypes of influenza A and influenza B, and compare the technology with single PCR. [Methods] H1 and H3 subtypes of influenza A and influenza B were used to design primers. Muhiplex PCR amplification and electrophoresis were performed, and its result was compared with that detected by single-PCR. [ Results] The multiplex PCR can detect three types of influenza viruses at the same time within 4 hours, but time was shorter than the single PCR, and the specificity and the sensitivity was similar to single PCR. [Conclusion] The detection system based on multiplex PCR was accurate, sensitive and specificity, which could be used rapidly to detect result.

关 键 词：多重PCR 流感病毒 

分 类 号：R373.13[医药卫生—病原生物学]