胰岛素增敏剂罗格列酮对链脲佐菌素损伤SH-SY5Y细胞的保护作用  

Protective Effect of Insulin Sensitizer Rosiglitazone on SH-SY5Y Cell against Streptozotocin-induced Injury

作　　者：张景燕[1] 王蓉[1] 盛树力[1] 姬志娟[1] 孟祥宏[1] 赵志炜[1] 

机构地区：[1]首都医科大学宣武医院中心实验室,神经变性病教育部重点实验室

出　　处：《首都医科大学学报》2010年第1期69-71,共3页Journal of Capital Medical University

基　　金：国家高技术研究发展计划(863计划)(2006AA02A245);北京市自然科学基金(7082043);首都医学发展科研基金(2007-3108);北京市卫生系统高层次卫生技术人才计划(2009-3-64)资助项目~~

摘　　要：目的观察胰岛素增敏剂罗格列酮对链脲佐菌素(streptozotocin,STZ)所致胰岛素信号转导通路损伤的SH-SY5Y细胞的影响。方法将人神经母细胞瘤株SH-SY5Y细胞分为对照组、STZ制备神经元损伤模型组(STZ 0.8 mmol/L)、罗格列酮干预组(STZ 0.8 mmol/L+罗格列酮20μmol/L),测定每组的细胞计数、噻唑蓝(MTT)代谢率、乳酸脱氢酶(LDH)漏出率,观察罗格列酮对SH-SY5Y细胞的作用。结果与对照组相比,STZ模型组的细胞计数和MTT代谢率降低(P<0.01),LDH漏出率升高(P<0.01);经罗格列酮保护后,上述细胞生存指标明显改善,细胞计数和MTT代谢率均高于STZ损伤组(P<0.01),LDH漏出率降低(P<0.01)。结论罗格列酮可以减轻STZ引起的神经细胞毒性,提高细胞生存率,其作用机制尚需进一步探讨。Objective To investigate the effect of insulin sensitizer rosiglitazone on streptozotocin（STZ）induced insulin signal transduction pathway injury in SH-SY5Y cells. Methods Cells from human neuroblastoma cell line SH-SY5Y were divided into control group, STZ 0.8 mmol/L group, STZ 0.8 mmol/L＋rosiglitazone 20 μmol/L group. The cells were counted, MTT（MTT） metabolic rate, lactate dehydrogenase（LDH） leakage rate in each group were measured, and the effect of rosiglitazone on SH-SY5Y cells was observed. Results Compared with the control group, cell count and MTT metabolic rate of STZ model decreased（P〈0.01）, and LDH leakage rate increased（P〈0.01）. After rosiglitazone protection, these indicators were significantly improved, cell count and MTT metabolic rate were higher than that in STZ injury group（P〈0.01）, LDH leakage rate was lower（P〈0.01）. Conclusion Rosiglitazone can reduce STZ-induced nerve cell toxicity, to improve cell survival, though its mechanism still need to be further explored.

关 键 词：人神经母细胞瘤株SH—SY5Y 链脲佐菌素 罗格列酮 

分 类 号：R338.1[医药卫生—人体生理学]