载牛血清蛋白的PLGA纳米粒制备工艺的优化及特性研究  

Optimization of preparation and characteristic of BSA-PLGA nanoparticles

作　　者：梅林[1] 龙大宏[1] 黄婉丹[1] 刘菲菲[1] 许孟杰[1] 

机构地区：[1]广州医学院人体解剖学教研室,广东广州510182

出　　处：《解剖学研究》2010年第1期1-5,共5页Anatomy Research

基　　金：广东省自然科学基金(9151063101000016)

摘　　要：目的制备载牛血清蛋白(BSA)的PLGA纳米粒(NPs),采用正交试验设计对工艺进行优化筛选,并研究其特性。方法以聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]为载体,二氯甲烷(DCM)和丙酮为有机溶剂,采用复乳化溶剂挥发法制备载BSA的PLGA载药纳米粒。扫描电镜观察纳米粒形态,纳米粒度分析仪测定平均粒径和粒径分布;BCA法测定纳米粒的包封率;同时考察其体外释放特性。结果优化条件下制备的纳米粒呈大小均匀的球形粒子,平均粒径为219nm,包封率为44.7%;体外释放分初期突释和后期缓释两阶段,其2～28d的释放曲线符合Higuchi方程,28d末的累积释放量为87.37%。结论以PLGA为载体的BSA纳米粒具有较小的粒径、较高包封率和明显的缓释性能。[Abstract] Objective To prepare the bovine serum albumin （BSA） loaded PLGA nanoparticles and to optimize the for- mulations of nanoparticles by design of orthogonality and study their characteristics. Methods Nanoparticales were prepared by a double emulsification solvent evaporation method with poly（lactic-co-glycolic） acid （PLGA） as carrier material and methylene chloride （DCM） and acetone as organic solvent. Scanning electron microscopy SEM） was used for the observation of nanoparti- cles. Mean diameter and particle size distribution were determined by photon correlation spectroscopy （PCS）. The encapsulation efficiency of BSA in the nanoparticles was evaluated by BCA method, which was also performed for the release of BSA from nanoparticles. Results Scanning electron microscopy （SEM） showed the nanoparticles which prepared by optimal preparation were the size of uniform spherical particles. Mean diameter and encapsulation efficiency of nanoparticles were 219 nm and 44.7% respectively. The BSA released from nanoparticles appeared to be consistent with initial rapid-release and later slow-re- lease. The releasing in vitro was consistent with Higuchi model. 87.37% Encapsulated BSA was released over a priod of 28 d. Conclusion The BSA-loaded PLGA nanoparticles are found to be a smaller particle size, a good encapsulation efficiency and markably sustained release of BSA.

关 键 词：牛血清蛋白 聚乳酸-羟基乙酸共聚物 正交试验 纳米粒 

分 类 号：R318.08[医药卫生—生物医学工程]