基于16S rDNA基因的中国部分地区非B型烟粉虱系统发育关系分析  

Phylogenetic analysis of Bemisia tabaci non-B biotypes in partial areas in China based on 16S rDNA gene

作　　者：沈媛[1] 杜予州[1] 金桂华[2] 邱宝利[2] 郑福山[1,2] 任顺祥[2] 

机构地区：[1]扬州大学应用昆虫研究所,扬州225009 [2]华南农业大学资源环境学院昆虫学系/教育部生物防治工程中心,广州510642

出　　处：《昆虫学报》2010年第1期82-90,共9页Acta Entomologica Sinica

基　　金：公益性行业科研专项--粉虱类害虫可持续治理技术研究与集成示范(200803005);国家科技支撑计划项目(2006BAD08A08);江苏省科技攻关项目(BE2005348)

摘　　要：【目的】烟粉虱Bemisia tabaci是一个快速进化的复合种。由于近年来烟粉虱危害不断增加,其生物型问题也越来越受到关注。在我国,不仅存在危害严重的B型烟粉虱,同时也已发现多种非B型烟粉虱。通过鉴定我国部分地区目前发生的烟粉虱生物型种类,分析它们与世界各地不同生物型之间的关系,可为我国烟粉虱的生物型检测和综合防治提供依据。【方法】利用16SrDNA基因作为分子标记,鉴定了2005年与2006年在我国6个省份(自治区)采集的22个非B型烟粉虱样本的生物型,并探讨了各生物型之间的系统进化关系。【结果】所研究的22个非B型烟粉虱归属于Q型、Nauru型和An型,3种生物型之间的遗传距离在10%以上,但是Q生物型与B生物型之间的亲缘关系最近,遗传距离在2.8%～4.0%范围内;进化分歧数据还表明,不同生物型之间的遗传距离明显大于同一生物型内遗传距离,其中Q型内部差异最小,在0.9%以内;同时结果表明目前在我国多个生物型共同存在是一个普遍的现象,其中Nauru型的分布较广泛;在云南地区检测到的非B型烟粉虱生物型类型最多。【结论】烟粉虱生物型遗传分化复杂,利用16S rDNA基因能有效鉴定烟粉虱的生物型。[Aim]Bemisia tabaci is a species complex with rapid evolutionary modifications. In recent years,its biotypes gained more attention because of its increasing hazards to the agricultural crops. Previously there were few sympatric non-B biotypes in different regions of China except B biotype. The study described herein provides some theoretical basis for its biotype detection and integrated control by identifying its biotypes which currently prevail in China and their relationship with different biotypes from other countries.[Methods]The biotypes and phylogenetic relationships of 22 different non-B biotype populations collected from six provinces were studied based on 16S rDNA sequences. [Results] The results revealed that the 22 different populations of non-B B. tabaci belong to Q,Nauru and An biotypes. Evolutionary divergence among these biotypes was more than 10%. The relationship between Q and B biotypes was closest,with genetic distances between 2.8% and 4.0%. The genetic distances among different biotypes were significantly higher than within the same biotype,while Q biotype showed the least genetic distance of 0.9% within itself. It is a universal phenomenon that multiple biotypes prevail in the same region. The Nauru biotype was more widely distributed than the other two biotypes. The highest variety of non-B biotypes was detected in Yunnan province. [Conclusion] The genetic differentiation of B. tabaci is very complicated. The biotypes of B. tabaci can be effectively identified using 16S rDNA gene.

关 键 词：烟粉虱 生物型 16S RDNA 遗传距离 系统发育 

分 类 号：Q968[生物学—昆虫学]