电镜重构病毒的三维显示与三维任意区域分割  

Three-dimensional representation and arbitrary region segmentation in 3D reconstruction of virus particles by electron cryo-microscopy

作　　者：李晶 赵海燕[2] 李鲲鹏[3] 张景强[3] 

机构地区：[1]昆明总医院医学工程科,云南昆明650032 [2]云南省第一人民医院眼科,云南昆明650032 [3]中山大学生命科学学院,广东广州510275

出　　处：《电子显微学报》2009年第6期528-532,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.30770540);成都军区医学科研计划课题(No.MB07026)~~

摘　　要：利用冷冻电镜单颗粒三维重建方法获得分辨率1.5nm家蚕质多角体(BmCPV);2.5nm,中蜂囊状幼虫病病毒(CSBV);1.4nm,伊蚊C6/36细胞浓核病毒(C6/36DNV)的三维结构体数据,对三维结构数据进行了数据归并,并找到对显示细节较为敏感的向量维度,用表面显示方法对病毒体数据进行三维显示,用基于空间的方法完成了任意区域的交互分割。显示时间和信噪比与原有显示方法有了提高。病毒表面细节和轴上突起清晰可见。所分割的部分结构准确完整,并可以对所分割的部分进行量化分析。为冷冻电镜单颗粒重构提供了新的三维可视与分析方法。Recent advances in electron cryomicroscopic instrumentation and single particle reconstruction method have created opportunities for high-throughput and high-resolution three-dimensional（3D）structure determination of virus.In order to visual and understand effectively these 3D structure,here,we present a display method based on surface rendering with the function of 3D arbitrary region interactive segmentation and quantitative analysis,and integration them into software package CEM-3DDS（electron cryo-microscopy three-dimensional virus display and arbitrary region segmentation system）.CEM-3DVDDSS consists of a complete set of modular programs for icosahedral virus 3D representation and segmentation organized under a graphical user interface and provides options for user-friendly.Firstly,we translate volume data form format MRC obtaining from electron cryo-microscopy single particle reconstruction to format fitting our software.In the processing,the original volume data is merged for compression data and a better vector dimension is found for controlling detail resolution in the data translation.Then,the new volume data can be displayed and segmented using CEM-3DVDDSS.We demonstrate the applicability of CEM-3DVDDSS by using it to display 1.5nm（resolution）BmCPV（Bombyx mori Cytoplasmic Polyhedrosis Virus）,2.5 nmCSBV（Chinese Sacbrood Bee Virus）,and 1.4 nm C6/36DNV（Bensonucleosis Virus）3D structure.As a results,both 3D display speed and signal to noise ratio are improved comparing to original method,and segmentation results are exact and whole with additive function of quantitative analysis to 3D structure.

关 键 词：冷冻电子显微技术 三维显示 三维分割 病毒 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]