盾叶薯蓣类原球茎的细胞结构及薯蓣皂苷的组织化学定位  

Studies on cytoarchitecture feature and histochemistry of Protocorm-like body of Dioscorea zingiberensis C. H. Wright

作　　者：彭晓英[1] 周朴华[1] 蒋道松[1] 王晶莹[2] 赵黎明[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]吉林大学植物科学学院,吉林长春130062

出　　处：《电子显微学报》2009年第6期579-584,共6页Journal of Chinese Electron Microscopy Society

基　　金：湖南省重点科技攻关项目资助(编号:BK0272)

摘　　要：为揭示盾叶薯蓣类原球茎发育的细胞学基础和薯蓣皂苷在各离体培养繁殖体中的分布,应用石蜡切片技术、扫描电镜和透射电镜制片技术及组织化学方法进行了研究。制片观察结果显示:盾叶薯蓣类原球茎是由多层鳞片叶包裹的致密小球体,从外至内由周皮、基本组织和分散在基本组织中的维管束组成,初生增厚分生组织和原形成层是其增大的主要原因之一;组织化学结果显示:愈伤组织和类原球茎中均有薯蓣皂苷的分布,而表皮栓质化的愈伤组织和生根类原球茎中薯蓣皂苷的含量相对较多。By using the techniques of light microscope,scanning electron microscope,transmission electron microscope and histochemical localization of dioscin,the cytoarchitecture feature and dioscin distribution of the protocorm-like body（PLB）of Dioscorea zingiberensis C.H.Wright were studied.The results showed the PLB,a compact corm wrapped by scale leaves,consisted of periderm and vascular bundle scattered among the ground tissue,PLB enlargement largely due to the cell division of the primary thicking meristem（PTM）and procambium.Furthermore,callus with suberized epidermis and rooted PLB stained more intensely than the tenders,which indicated more diosgenin located within the old tissues in vitro.

关 键 词：盾叶薯蓣 细胞结构 组织化学 薯蓣皂苷元 类原球茎 电镜 

分 类 号：S567.239[农业科学—中草药栽培]