特异性免疫治疗对哮喘大鼠转化生长因子-β_1/Smad信号通路的影响  

Effect of specific immunotherapy on TGF-β_1/Smads in asthmatic rats

作　　者：李绍波[1] 金小红[1] 王昕昕[1] 赖小英[1] 童夏生[2] 

机构地区：[1]台州医院儿内科 [2]台州中西医结合医院儿内科

出　　处：《中国临床药理学与治疗学》2009年第11期1229-1233,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省医药卫生科技计划(2008B192);台州市科技局课题(061KY06)

摘　　要：目的:探讨特异性免疫治疗(specific immunotherapy,SIT)对哮喘大鼠转化生长因子-β1(TGF-β1)及信号转导分子Smads表达的影响。方法:40只健康雄性清洁级Wistar大鼠随机均分成正常对照组、哮喘组、SIT对照组和SIT治疗组等4组,每小组10只。通过卵蛋白(OVA)雾化吸入的方法对致敏大鼠进行SIT,用双抗体夹心ELISA法测定血清和BALF中TGF-β1浓度,免疫组化方法检测肺组织TGF-β1以及磷酸化Smad2/3(P-Smad2/3)、Smad7蛋白表达水平。结果:哮喘组和SIT治疗组的血清、BALF中TGF-β1浓度均分别高于正常对照组,但SIT治疗组与哮喘组相比,两者的TGF-β1浓度则均有所下降;哮喘组肺组织TGF-β1和P-Smad2/3蛋白表达较正常对照组和SIT治疗组增高,而Smad7蛋白表达下降。结论:SIT通过抑制哮喘大鼠体内TGF-β1和P-Smad2/3的过度表达及上调Smad7,从而阻断TGF-β1的胞内信号转导,可在一定程度上减轻哮喘大鼠气道炎症和抑制气道重塑的发生发展。AIM：To approach the effect of specific immunotherapy on transforming growth factor （TGF-β1） /Smads in asthmatic rats. METHODS： Forty Wistar rats were randomly divided into 4 groups： control group, asthma group, control of specific immunotherapy group and specific immunotherapy group, with 10 rats in each group. The model of asthma was establishe by the ovalbumin （OVA） challenge methods and the specific immunotherapy group received specific immunotherapy by repeated inhaling different doses of OVA. The concentrations of TGF-β1 in BALF and serum were detected by EHSA. The expressions of TGF-β1, phosphorylated Smad2/3 （ P-Smad2/3 ） and Smad7 proteins in lung tissue were detected by immunocytochemical method. RESULTS：In BALF or senma, the concen- tration of TGF-β1 in control group was lower than that in asthma group or specific immunotherapy group respectively. The concentrations of TGF-β1 in BALF or serum in specific immunotherapy group was lower than that in asthma group. The expressions of TGF-β1 and P- Smad2/3 in lung in asthma group were higher, but the expressions of Smad7 was lower than that in control group or in specific immunotherapy group respectively. CONCLUSION： Specific immunotherapy could decrease the expressions of TGF-β1 and P-Smad2/3, while increase the expression of Smad7 in asthmatic rat, and relieve the airway inflammation in some extent and inhibit the occurrence and development of airway remodeling.

关 键 词：哮喘 特异性免疫治疗 转化生长因子-Β1 SMADS 

分 类 号：R965.2[医药卫生—药理学]