小鼠闭合性股骨骨折模型的建立与评估  

Establishing and evaluating of mouse closed femur fracture

作　　者：张连方[1] 齐进[1] 王晋申[1] 钱念东[1] 周琦[1] 杨军[2] 邓廉夫[1] 

机构地区：[1]上海市伤骨科研究所上海交通大学医学院附属瑞金医院骨科,上海200025 [2]上海交通大学Bio-X中心,上海200030

出　　处：《中国矫形外科杂志》2010年第2期143-146,共4页Orthopedic Journal of China

基　　金：国家自然科学基金(编号:30672145;30872641);上海市科委研发基地基金(编号:08DZ2200500)

摘　　要：[目的]建立小鼠标准闭合性股骨骨折模型。[方法]将60只C57BL/6雄性小鼠的右侧股骨髓腔插入0.45 mm直径的钢针,然后通过自行设计的骨折造模支架造成股骨中段闭合骨折。通过影像学、组织学方法观测骨折愈合进程。[结果]术后通过X线证实横型骨折55例,建模成功率为91.67%。X线、Micro-CT和组织学检查表明骨折愈合过程为典型的Ⅱ期愈合过程。[结论]通过自制骨折模型支架制作的小鼠闭合性股骨骨折模型操作简便、重复性高,其愈合为典型Ⅱ期愈合过程,可用于骨折愈合机制的研究。[ Objective ] To develop a standard closed experimental fracture model in mouse femur. [ Method ] A 0.45 mm stainless-steel rod was introduced into the medullary cavity and the pre-nailed femur shaft was fractured by an impact device, which resulted in a reproducible transverse fracture pattern. The fractures were examined using X-ray Micro-CT,and histological method. [ Result] Transverse fractures were created in 91.67% of the experimental mice, and the fracture healing was typical secondary fracture healing. [ Conclusion ] The standard closed fracture model in this experiment, whose healing was typically secondary fracture healing, can be obtained easily and used in study of fracture.

关 键 词：小鼠 造模支架 闭合性股骨骨折 

分 类 号：R683[医药卫生—骨科学] R-332[医药卫生—外科学]