NYGGF4基因在人前体脂肪细胞分化过程中的表达及肿瘤坏死因子α对其调控的研究  

Expression of the NYGGF4 gene during human preadipocyte differentiation and the regulative role of tumor necrosis factor-alpha

作　　者：赵亚萍[1] 王加林[1] 蔡友群 陈小慧[2] 张春梅[2] 郭锡熔[2] 

机构地区：[1]中国人民解放军第82医院内分泌科,江苏省淮安市223001 [2]南京医科大学附属南京妇幼保健院儿童保健科

出　　处：《中华医学遗传学杂志》2010年第1期69-72,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30571978,30973231）;江苏省医学领军人才项目（LJ200624）

摘　　要：目的观察人前体脂肪细胞诱导分化过程中NYGGF4基因mRNA表达水平的变化，探讨重组肿瘤坏死因子α（tumor necrosis factorα，TNFα）对成熟脂肪细胞中NYGGF4基因表达水平的调节作用。方法体外培养人内脏来源的原代前体脂肪细胞（human preadipocytes—visceral，HPA—v），在诱导HPA-v分化成熟的基础上，应用不同浓度重组TNFα干预成熟脂肪细胞16h，或以10ng／mL TNFα分别干预不同时间，采用实时荧光定量逆转录PCR技术检测的NYGGF4 mRNA表达水平。结果（1）HPA—v诱导分化至第17d，具备成熟脂肪细胞特征；（2）NYGGF4基因低表达于人前体脂肪细胞中，随脂肪细胞分化成熟其表达水平逐渐升高；（3）随TNFα干预浓度增高及干预时间延长，人成熟脂肪细胞中NYGGF4 mRNA水平呈现逐渐升高的趋势。结论NYGGF4基因具随脂肪细胞分化成熟表达逐渐上调的特征，TNFα能显著上调成熟脂肪细胞中NYGGF4基因的表达，其效应具有剂量依赖和时间反应性。Objective To observe the changes of NYGGF4 gene expression during human preadipocyte differentiation and to explore the regulative role of tumor necrosis factor α （TNFα） on NYGGF4 gene expression in matured human adipocytes. Methods Human preadipocytes-visceral （HPA- v） were cultured in vitro and differentiated into the matured adipocytes. Fully differentiated adipoeytes were treated with TNFα in different concentrations for 16 hours or at 10 ng/mL for various times. Levels of the NYGGF4 mRNA were evaluated by real-time reverse transcription-PCR（RT-PCR）. Results （1） After induction of differentiation, more than 90% of the HPAs-v exhibited typical adipocyte morphology on the 17th day. （2） In human preadipocytes, the level of NYGGF4 mRNA expression remained low. The NYGGF4 mRNA level was gradually increased with the preadipocytes being differentiated into the matured adipocytes, and reached the highest level in the fully differentiated adipocytes. （3） Treatment of the human matured adipoeytes with TNFα resulted in a significant increase in the level of NYGGF4 mRNA. The upregulatory effect of TNFα on the NYGGF4 gene expression tended to be enhanced with the increasing concentrations and elongation of time. Conclusion During human preadipoeytes differentiation, the level of NYGGF4 gene mRNA expression increases gradually. TNFα could upregulate the expression of NYGGF4 mRNA in human matured adipocytes. The regulatory effect of TNFα on the NYGGF4 gene expression is of dose dependent and time correlated.

关 键 词：NyGGF4基因 人前体脂肪细胞 细胞分化 肿瘤坏死因子Α 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]