结缔组织生长因子在幼年兔关节软骨全层缺损修复中的表达及意义  

Expression and its significance of connective tissue growth factor in articular cartilage during repair after full-thickness cartilage injury in young rabbit model

作　　者：付建军[1] 初同伟[1] 周跃[1] 刘玉刚[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《细胞与分子免疫学杂志》2010年第2期132-134,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30672120);重庆市自然科学基金资助项目(2006BB5355)

摘　　要：目的:探讨结缔组织生长因子(CTGF)在幼年兔膝关节软骨全层缺损修复过程中的表达及意义。方法:采用兔膝关节股骨关节面全层软骨缺损自我修复模型,25只幼年新西兰白兔,随机分为5组(每组5只):对照组、损伤24 h组、1周组、4周组、8周组。应用RT-PCR技术和免疫组化技术分别检测CTGF mRNA和蛋白在修复过程中的表达。结果:幼年兔关节软骨全层损伤后自我修复良好。各组检测均见CTGF mRNA表达,损伤后各组表达水平显著高于对照组(P<0.05)。免疫组化显示各组软骨细胞均有CTGF蛋白表达,阳性表达主要集中在软骨的中间带及深层带。结论:幼年兔关节软骨全层缺损自我修复过程伴有显著的CTGF表达上调,CTGF可能在关节软骨修复中发挥重要作用。AIM：To approach the expression and its significance of connection tissue growth factor in articular cartilage during repair after full-thickness cartilage injury in young rabbit model.METHODS：Young rabbit model of spontaneous healing of full-thickness cartilage defect in the medial femoral knee condyle was reproduced in present study.A total of twenty-five young New Zealand white rabbit were randomly divided into five groups：control group,24 hour group,1 week group,4 week group and 8 week group after full-thickness cartilage injury（5 each）.The mRNA and protein expression of CTGF were detected with RT-PCR and immunohistochemistry,respectively.RESULTS：The morphologically good repairs with hyaline-like cartilage appearance were observed at 8 weeks after injury.The expression of CTGF mRNA was detected in all groups.Expression of CTGF mRNA was significantly higher in all groups after cartilage injury than in control group（P〈0.05）.The immunohistochemistry results showed that the expression of CTGF was positive in all groups,and immunoloca-lization was observed in the middle and deep zone.CONCLUSION：The up-regulated expression of CTGF is accompanied with spontaneous healing of full-thickness cartilage defect in young rabbit,and CTGF may play a role in the rapair of articular cartilage injury.

关 键 词：结缔组织生长因子 关节软骨损伤 修复 

分 类 号：R681.3[医药卫生—骨科学] R392.1[医药卫生—外科学]