ASPH在肿瘤细胞和肿瘤组织中的分布及检测  

The distribution and expression pro-files of Aspartyl/Asparaginyl beta-hydroxylase(ASPH) in some tumorous cell lines and tissues

作　　者：宋凯[1] 薛小平[1] 王伟[1] 呼延霆[1] 汪桦[1] 杨慧[1] 谢琼[2] 

机构地区：[1]西北工业大学生命科学院,陕西西安710072 [2]中国航天员科研训练中心医监医保研究室,北京100094

出　　处：《细胞与分子免疫学杂志》2010年第2期141-144,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：西北工业大学基础科研基金资助项目(2003)

摘　　要：目的:检测肿瘤细胞系和我国肿瘤患者癌组织中天冬氨酰-(天冬酰胺酰)β-羟化酶(ASPH)的分布及表达谱。方法:采用RT-PCR法检测肿瘤细胞系中ASPH基因的转录水平;细胞免疫荧光和Western blot方法检测肿瘤细胞系中ASPH蛋白的表达水平;免疫组织化学方法检测人癌组织和正常组织中ASPH的分布与表达。结果:在肿瘤细胞系中AS-PH在转录和翻译水平均有不同程度表达,ASPH蛋白分布于细胞膜和胞质区域;在肿瘤患者组织中有ASPH的高表达,而人正常组织中不表达或表达量极低。结论:ASPH是一种有潜力的广谱性肿瘤标记分子,有可能用于肿瘤的早期检测。AIM：To investigate the distribution and expression profiles of Aspartyl/Asparaginyl beta-hydroxylase（ASPH） in some tumorous cell lines and some tissues.To evalue the distribution and ex-pression profile of Aspartyl/Asparaginyl beta-hydroxylase,ASPH in some tumor cell lines and tumor tissus.METHODS：The expression of ASPH in tumor cell lines on transcriptional level transcriptical level was tested by using RT-PCR,and on translational level was tested by cellular immunofluorescence and Western blot.The histologic sections from tumorous and normal tissue were tested immunohistochemistry.RESULTS：The expression of ASPH could be detected at both the transcriptional level and the translational level in the tumorous cell lines and it distributed in the cell membrane and the cytoplasmic domain.CONCLUSION：ASPH can be wildly used as a potential prognostic marker in neoplasms detection.

关 键 词：ASPH 肿瘤细胞系 人癌组织 标记分子 

分 类 号：Q291[生物学—细胞生物学]