体外不同动态力学应变对人牙周膜成纤维细胞整合素α5、α6及β1的表达影响  

Effect of different dynamic mechanical strain on the expression of integrins α5,α6,β1 in human periodontal ligament fibroblasts in vitro

作　　者：侯睿[1,2] 陈新民[2] 巢永烈[2] 李小玉[2] 

机构地区：[1]上海市徐汇区牙病防治所修复科,上海200032 [2]四川大学口腔疾病研究国家重点实验室,四川成都610041

出　　处：《细胞与分子免疫学杂志》2010年第2期172-174,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30700959)

摘　　要：目的:探讨体外培养的人牙周膜成纤维细胞(HP-DLF)在动态力学微应变范围内细胞膜表面整合素α5、α6、β1表达变化。方法:采用四点弯曲细胞力学加载装置对体外培养的HPDLF进行加载,通过流式细胞术(FCM)分析不同动态力学应变对HPDLF膜表面整合素α5、α6、β1表达的影响。结果:未加力条件下体外培养的HPDLF膜表面同时有整合素α5、α6、β1亚单位的表达;且β1的表达量最高,α5次之,α6较低;不同大小应变组和不同作用时间组的细胞膜表面整合素α5、α6、β1表达量之间均存在统计学差异(P<0.01),动态力学应变引起了HPDLF膜表面整合素α5、α6、β1表达的上调,此影响与应变的大小和作用时间密切相关;在不同亚单位间存在一定的相关性和差异性。结论:动态力学应变作用下整合素α5、α6、β1亚单位表达的上调,提示整合素β1亚族(特别是αβ亚单位结合体-α5β1和α6β1)可能在HP-DLF的力学信号转导中具有重要的调控作用。AIM：To study the expression of integrins α5,α6,β1 in human periodontal ligament fibroblasts（HPDLF） in response to dynamic mechanical strain of different magnitude and duration.METHODS：By means of four point bending system,the effect of dynamic mechanical strain of different magnitude and duration on the expression of integrins α5,α6,β1 on the membrane of HPDLF was investigated by flow cytometry（FCM）.RESULTS：There were actual expression of integrins α5,α6,β1 on the membrane of HPDLF without mechanical strain,with the highest expression of β1 subunit and lowest α6 subunit.Dynamic mechanical strain simultaneously up-regulated the expression of integrinsα5,α6,β1.Up-regulations of expression of three integrin subunits were varied to some extent,but all closely correlated with magnitude and duration of strain.CONCLUSION：These results suggest that integrins β1 subtribe,especially α5β1 and α6β1,possibly plays an important role in the regulation of mechanotransduction in HPDLF

关 键 词：牙周膜成纤维细胞 力学应变 流式细胞术 整合素 

分 类 号：R392.33[医药卫生—免疫学]